◆研究業績◆
(xx) Original Papers (English)
(Jxx) Original Papers (Japanese)
(Rxx) Reviews
(Pxx) Proceedings
(Bxx) Books
(BExx) Books (Editor)
(MPxx) Methods and Protocols
(Oxx) Others
1995
(1) Contribution of Hydrophobic Residues to the Stability of Human Lysozyme: Calorimetric Studies and
X-ray Structural Analysis of the Five Isoleucine to Valine Mutants
Kazufumi Takano, Kyoko Ogasahara, Hidetomo Kaneda, Yuriko Yamagata, Satoshi Fujii, Eiko
Kanaya, Masakazu Kikuchi, Motohisa Oobatake & Katsuhide Yutani
J. Mol. Biol. (1995) 254, 62-76.
1996
(2) The Structure, Stability, and Folding Process of Amyloidogenic Mutant Human Lysozyme
Jun Funahashi, Kazufumi Takano, Kyoko Ogasahara, Yuriko Yamagata & Katsuhide Yutani
J. Biochem. (1996) 120, 1216-1223.
1997
(3) Contribution of the Hydrophobic Effect to the Stability of Human Lysozyme: Calorimetric Studies and
X-ray Structural Analyses of the Nine Valine to Alanine Mutants
Kazufumi Takano, Yuriko Yamagata, Satoshi Fujii & Katsuhide Yutani
Biochemistry (1997) 36, 688-698.
(4) Contribution of Water Molecules in the Interior of a Protein to the Conformational Stability
Kazufumi Takano, Jun Funahashi, Yuriko Yamagata, Satoshi Fujii & Katsuhide Yutani
J. Mol. Biol. (1997) 274, 132-142.
1998
(5) A General Rule for the Relationship between Hydrophobic Effect and Conformational Stability of a
Protein: Stability and Structure of a Series of Hydrophobic Mutants of Human Lysozyme
Kazufumi Takano, Yuriko Yamagata & Katsuhide Yutani
J. Mol. Biol. (1998) 280, 749-761.
(6) Contribution of Hydrogen Bonds to the Conformational Stability of Human Lysozyme: Calorimetry and
X-ray Analysis of Six Tyr -> Phe Mutants
Yuriko Yamagata, Masahiro Kubota, Yohei Sumikawa, Jun Funahashi, Kazufumi Takano,
Satoshi Fujii & Katsuhide Yutani
Biochemistry (1998) 37, 9355-9362.
1999
(7) Contribution of Hydrogen Bonds to the Conformational Stability of Human Lysozyme: Calorimetry and
X-ray Analysis of Six Ser -> Ala Mutants
Kazufumi Takano, Yuriko Yamagata, Masahiro Kubota, Jun Funahashi, Satoshi Fujii &
Katsuhide Yutani
Biochemistry (1999) 38, 6623-6629.
(8) Contribution of Amino Acid Substitutions at Two Different Interior Positions to the Conformational
Stability of Human Lysozyme
Jun Funahashi, Kazufumi Takano, Yuriko Yamagata & Katsuhide Yutani
Protein Engng. (1999) 12, 841-850.
(9) Contribution of Intra- and Intermolecular Hydrogen Bonds to the Conformational Stability of Human
Lysozyme
Kazufumi Takano, Yuriko Yamagata, Jun Funahashi, Yusaku Hioki, Seiki Kuramitsu &
Katsuhide Yutani
Biochemistry (1999) 38, 12698-12708.
(10) Experimental Verification of the "Stability Profile of Mutant Protein" (SPMP) Data Using Mutant
Human Lysozymes
Kazufumi Takano, Motonori Ota, Kyoko Ogasahara, Yuriko Yamagata, Ken Nishikawa &
Katsuhide Yutani
Protein Engng. (1999) 12, 663-672.
(11) Effect of Foreign N-terminal Residues on the Conformational Stability of Human Lysozyme
Kazufumi Takano, Kimiko Tsuchimori, Yuriko Yamagata & Katsuhide Yutani
Eur. J. Biochem. (1999) 266, 675-682.
(R1) 蛋白質立体構造の安定化因子をどのように見積もればよいか
高野和文、舩橋順、油谷克英
生物物理 (1999) 39, 92-96.
(R2) Evaluation of Some Factors that Contribute to Conformational Stability of a Protein Using Database
of Stability/Structure
Katsuhide Yutani, Kazufumi Takano & Jun Funahashi
Old and New Views of Protein Folding (K. Kuwajima & M. Arai, eds.) (1999) pp. 175-184, Elsevier
Science, Amsterdam.
2000
(12) Amyloid Protofilament Formation of Hen Egg Lysozyme in Highly Concentrated Ethanol Solution
Shuichiro Goda, Kazufumi Takano, Yuriko Yamagata, Ryou Nagata, Hideo Akutsu, Saori Maki,
Keiichi Namba & Katsuhide Yutani
Protein Sci. (2000) 9, 381-387.
(13) Effect of Extra N-terminal Residues on the Stability and Folding of Human Lysozyme Expressed in
Pichia pastoris
Shuichiro Goda, Kazufumi Takano, Yuriko Yamagata, Yoshio Katakura & Katsuhide Yutani
Protein Engng. (2000) 13, 299-307.
(14) Role of Amino Acid Residues at Turns in the Conformational Stability and Folding of Human Lysozyme
Kazufumi Takano, Yuriko Yamagata & Katsuhide Yutani
Biochemistry (2000) 39, 8655-8665.
(15) Contribution of Salt Bridges near the Surface of a Protein to the Conformational Stability
Kazufumi Takano, Kimiko Tsuchimori, Yuriko Yamagata & Katsuhide Yutani
Biochemistry (2000) 39, 12375-12381.
(16) Role of Surface Hydrophobic Residues in the Conformational Stability of Human Lysozyme at Three
Different Positions
Jun Funahashi, Kazufumi Takano, Yuriko Yamagata & Katsuhide Yutani
Biochemistry (2000) 39, 14448-14456.
2001
(17) The Stability and Folding Process of Amyloidogenic Mutant Human Lysozymes
Kazufumi Takano, Jun Funahashi & Katsuhide Yutani
Eur. J. Biochem. (2001) 268, 155-159.
(18) Are the Parameters of Various Stabilization Factors Estimated from Mutant Human Lysozymes Compatible
with Other Proteins?
Jun Funahashi, Kazufumi Takano & Katsuhide Yutani
Protein Engng. (2001) 14, 127-134.
(19) Contribution of Polar Groups in the Interior of a Protein to the Conformational Stability
Kazufumi Takano, Yuriko Yamagata & Katsuhide Yutani
Biochemistry (2001) 40, 4853-4858.
(20) Role of Non-glycine Residues in Left-handed Helical Conformation for the Conformational Stability of
Human Lysozyme
Kazufumi Takano, Yuriko Yamagata & Katsuhide Yutani
Proteins (2001) 44, 233-243.
(21) A New Scale for Side Chain Contribution to Protein Stability Based on the Empirical Stability
Analysis of Mutant Proteins
Kazufumi Takano & Katsuhide Yutani
Protein Engng. (2001) 14, 525-528.
(22) Role of Amino Acid Residues in Left-handed Helical Conformation for the Conformational Stability of
a Protein
Kazufumi Takano, Yuriko Yamagata & Katsuhide Yutani
Proteins (2001) 45, 274-280.
(R3) Stability and Structure of a Series of Mutant Human Lysozymes
Kazufumi Takano & Katsuhide Yutani
Curr. Topics in Peptide & Protein Res. (2001) 4, 1-16.
2002
(23) Positive Contribution of Hydration Structure on the Surface of Human Lysozyme to the Conformational
Stability
Jun Funahashi, Kazufumi Takano, Yuriko Yamagata & Katsuhide Yutani
J. Biol. Chem. (2002) 277, 21792-21800.
(24) Elongation in a β-Structure Promotes Amyloid-like Fibril Formation of Human Lysozyme
Shuichiro Goda, Kazufumi Takano, Yuriko Yamagata, Saori Maki, Keiichi Namba & Katsuhide
Yutani
J. Biochem. (2002) 132, 655-661.
(25) Promotion of Large Protein Crystal Growth with Stirring Solution
Hiroaki Adachi, Kazufumi Takano, Masashi Yoshimura, Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2002) 41, L1025-L1027.
(J1) 有機およびタンパク質結晶育成における短光パルスレーザーの効果
安達宏昭、細川陽一郎、高野和文、常定扶美、増原宏、吉村政志、森勇介、佐々木孝友
日本結晶成長学会誌 (2002) 29, 445-449.
2003
(26) Buried Water Molecules Contribute to the Conformational Stability of a Protein
Kazufumi Takano, Yuriko Yamagata & Katsuhide Yutani
Protein Engng. (2003) 16, 5-9.
(27) Application of a Two-liquid System to Sitting-drop Vapor Diffusion Protein Crystallization
Hiroaki Adachi, Kazufumi Takano, Masaaki Morikawa, Shigenori Kanaya, Masashi Yoshimura,
Yusuke Mori & Takatomo Sasaki
Acta Cryst. (2003) D53, 194-196.
(28) Application of a Stirring Method to Micro-scale and Vapor Diffusion Protein Crystallization
Hiroaki Adachi, Kazufumi Takano, Masashi Yoshimura, Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2003) 42, L314-L315.
(29) Effective Protein Crystallization Using Crystal Hysteresis
Hiroaki Adachi, Kazufumi Takano, Masashi Yoshimura, Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2003) 42, L384-L385.
(O1) カルチャーショック、そして新たな取り組みへ
高野和文
生産と技術 (2003) 55, 19-21.
(R4) 蛋白質結晶成長における新しい手法とそのメカニズム−結晶工学からのアプローチ−
安達宏昭 & 高野和文
熱測定 (2003) 30, 148-149.
(30) Laser Irradiated Growth of Protein Crystal
Hiroaki Adachi, Kazufumi Takano, Youichiroh Hosokawa, Tsuyoshi Inoue, Yusuke Mori,
Hiroyoshi Matsumura, Masashi Yoshimura, Yasuo Tsunaka, Masaaki Morikawa, Shigenori Kanaya,
Hiroshi Masuhara, Yasushi Kai & Takatomo Sasaki
Jpn. J. Appl. Phys. (2003) 42, L798-L800.
(31) The Contribution of Polar Group Burial to Protein Stability is Strongly Context Dependent
Kazufumi Takano, J. Martin Scholtz, James C. Sacchettini & C. Nick Pace
J. Biol. Chem. (2003) 278, 31790-31795.
(32) New Practical Technique for Protein Crystallization with Floating and Stirring Methods
Hiroaki Adachi, Hiroyoshi Matsumura, Kazufumi Takano, Ai Niino, Tsuyoshi Inoue,
Masashi Yoshimura, Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2003) 42, L1161-L1163.
2004
(33) Two-Liquid Hanging-Drop Vapor-Diffusion Technique of Protein Crystallization
Hiroaki Adachi, Hiroyoshi Matsumura, Kazufumi Takano, Ai Niino, Tsuyoshi Inoue,
Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, L79-L81.
(34) Protein Crystal Processing Using a Deep-UV Laser
Hiroshi Kitano, Hiroaki Adachi, Atsutoshi Murakami, Hiroyoshi Matsumura, Kazufumi Takano,
Tsuyoshi Inoue, Yusuke Mori, Soichi Owa & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, L73-L75.
(35) A Semiautomatic Protein Crystallization System with Preventing Evaporation of Drops and
Surface Sensor of Solution
Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Ai Niino, Takeshi Ishizu,
Tsuyoshi Inoue, Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, L76-L78.
(36) Protein Crystal Growth with a Two-Liquid System and Stirring Solution
Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Tsuyoshi Inoue, Yusuke Mori
& Takatomo Sasaki
J. Synchrotron Rad. (2004) 11, 121-124.
(37) Pre-Stirring Promotes Nucleation of Protein Crystals
Hiroaki Adachi, Ai Niino, Hiroyoshi Matsumura, Kazufumi Takano, Tsuyoshi Inoue,
Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, L243-L246.
(38) New Approach to Improve X-Ray Diffraction Pattern of Protein Crystal Using UV-Laser
Ablative Processing
Hiroshi Kitano, Hiroaki Adachi, Atsutoshi Murakami, Hiroyoshi Matsumura, Kazufumi Takano,
Tsuyoshi Inoue, Yusuke Mori, Soichi Owa & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, L297-L299.
(R5) Amyloidfibrils from the Viewpoint of Protein Folding
Satoshi Ohnishi & Kazufumi Takano
Cell. Mol. Life Sci. (2004) 61, 511-524.
(39) Improving the Quality of Protein Crystals Using a Stirring Crystallization
Hiroaki Adachi, Hiroyoshi Matsumura, Ai Niino, Kazufumi Takano, Takayoshi Kinoshita,
Masaichi Warizaya, Tsuyoshi Inoue, Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, L522-L525.
(40) Possible Involvement of a FKBP Family Protein from a Psychrotrophic Bacterium
Shewanella sp. SIB1 in Cold-Adaptation
Yutaka Suzuki, Mitsuru Haruki, Kazufumi Takano, Masaaki Morikawa & Shigenori Kanaya
Eur. J. Biochem. (2004) 271, 1372-1381.
(B1) タンパク質の安定性とフォールディング
高野和文
ゲノミクス・プロテオミクスの新展開（今中忠行監修）、エヌティーエス出版 (2004) 701-705.
(41) Generation of Protein Crystals Using a Solution-Stirring Technique
Hiroaki Adachi, Ai Niino, Hiroyoshi Matsumura, Kazufumi Takano, Takayoshi Kinoshita,
Masaichi Warizaya, Tsuyoshi Inoue, Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, L762-L764.
(42) The Effects of Solution Stirring on Protein Crystal Growth
Mari Yaoi, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Tsuyoshi Inoue,
Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, L686-L688.
(43) Mutational and Structural-based Analyses of the Osmolyte Effect on Protein Stability
Kazufumi Takano, Minoru Saito, Masaaki Morikawa & Shigenori Kanaya
J. Biochem. (2004) 135, 701-708.
(44) Universal Processing Technique for Protein Crystals Using a Pulsed UV Laser
Atsutoshi Murakami, Hiroshi Kitano, Hiroaki Adachi, Hiroyoshi Matsumura, Kazufumi Takano,
Tsuyoshi Inoue, Yusuke Mori, Masaaki Doi & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, L873-L876.
(R6) レーザー照射による蛋白質結晶成長制御
安達宏昭、高野和文、松村浩由、井上豪、森勇介、佐々木孝友
日本結晶学会誌 (2004) 46, 238-242.
(O2) 創晶プロジェクト
安達宏昭、松村浩由、村上聡、高野和文、井上豪、森勇介、佐々木孝友
日本結晶成長学会誌 (2004) 31, 34-35.
(R7) 紫外レーザーを用いた蛋白質結晶の加工
北野博史、安達宏昭、松村浩由、高野和文、井上豪、森勇介、佐々木孝友
日本結晶学会誌 (2004) 46, 292-296.
(45) Novel and Simple Screening Techniques of Protein Crystallization by Vapor Diffusion Rate Control
Hiroaki Adachi, Ai Niino, Hiroyoshi Matsumura, Kazufumi Takano, Tsuyoshi Inoue,
Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, 6264-6267.
(46) Gene Cloning and Biochemical Characterizations of a Thermostable Ribonuclease HIII from
Bacillus stearothermophilus
Hyongi Chon, Rikita Nakano, Naoto Ohtani, Mitsuru Haruki, Kazufumi Takano,
Masaaki Morikawa & Sigenori Kanaya
Biosci. Biotechnol. Biochem. (2004) 68, 2138-2147.
(47) Cleavage of Various Peptides with Pitrilysin from Escherichia coli: Kinetic Analyses Using
β-Endorphin and its Derivatives
Joel Cornista, Satoshi Ikeuchi, Mitsuru Haruki, Atsuko Kohara, Kazufumi Takano,
Masaaki Morikawa & Sigenori Kanaya
Biosci. Biotechnol. Biochem. (2004) 68, 2128-2137.
(48) Application of UV-Laser Ablation to Detaching Protein Crystal from Growth Vessel
Hiroshi Kitano, Hiroaki Adachi, Atsushi Sato, Atsutoshi Murakami, Hiroyoshi Matsumura,
Kazufumi Takano, Tsuyoshi Inoue, Yusuke Mori, Masaaki Doi & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, L1271-L1274.
(49) Effect of Stirring Method on Protein Crystallization
Mari Yaoi, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Tsuyoshi Inoue,
Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, L1318-L1319.
(50) Membrane Protein Crystallization Using Laser Irradiation
Hiroaki Adachi, Satoshi Murakami, Ai Niino, Hiroyoshi Matsumura, Kazufumi Takano,
Tsuyoshi Inoue, Akihito Yamaguchi, Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, L1376-L1378.
(R8) 創晶プロジェクト‐新しいタンパク質結晶育成技術の開発‐
安達宏昭、新納愛、北野博史、村上聡、高野和文、松村浩由、井上豪、森勇介、佐々木孝友
構造生物 (2004) 10, 41-58.
(51) Kinetically Robust Monomeric Protein from a Hyperthermophile
Atsushi Mukaiyama, Kazufumi Takano, Mitsuru Haruki, Masaaki Morikawa & Sigenori Kanaya
Biochemistry (2004) 43, 13859-13866.
(52) Control of Protein Crystal Nucleation and Growth Using Stirring Solution
Ai Niino, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Takayoshi Kinoshita,
Masaichi Warizaya, Tsuyoshi Inoue, Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2004) 43, L1442-L1444.
(R9) 生物工学におけるバイオクリスタルデザインの幕開け
高野和文
生物工学会誌 (2004) 82, 552.
(P1) Novel Approach to Process Protein Crystals using Deep-UV Laser
Hiroshi Kitano, Atsushi Sato, Atsutoshi Murakami, Hiroaki Adachi, Hiroyoshi Matsumura,
Kazufumi Takano, Tsuyoshi Inoue, Yusuke Mori, Masaaki Doi & Takatomo Sasaki
Proceedings of SPIE (2004) 5662, 280-284.
2005
(53) Isolation and Characterization of Rhodococcus sp. TMP2 and T12 that Degrade
2,6,10,14-Tetramethylpentadecane (Pristane) at Moderately Low Temperatures
Namio Kunihiro, Mitsuru Haruki, Kazufumi Takano, Masaaki Morikawa & Sigenori Kanaya
J. Biotechnol. (2005) 115, 129-136.
(54) Stabilities and Activitiesof the N- and C-domains of FKBP22 from a Psychrotrophic Bacterium
Overproduced in E. coli
Yutaka Suzuki, Kazufumi Takano & Shigenori Kanaya
FEBS J. (2005) 272, 632-642.
(R10) バイオクリスタルデザイン
高野和文、安達宏昭、松村浩由、井上豪、森勇介
生物物理 (2005) 45, 37-40.
(55) Gene Cloning, Overproduction, and Characterizations of Thermolabile Alkaline Phosphatase from
a Psychrotrophic Bacterium
Yutaka Suzuki, Yoichi Mizutani, Tadao Tsuji, Naoto Ohtani, Kazufumi Takano
Mitsuru Haruki, Masaaki Morikawa & Shigenori Kanaya
Biosci. Biotechnol. Biochem. (2005) 69, 364-373.
(56) Identification of Single Mn2+ Binding Sites Required for Activation of the Mutant Proteins of
E. coli RNase HI at Glu48 and/or Asp134 by X-ray Crystallography
Yasuo Tsunaka, Kazufumi Takano, Hiroyoshi Matsumura, Yuriko Yamagata & Shigenori Kanaya
J. Mol. Biol. (2005) 345, 1171-1183.
(57) Protein Cryocrystallography Using Laser-processed Crystal
Hiroshi Kitano, Hiroyoshi Matsumura, Hiroaki Adachi, Satoshi Murakami, Kazufumi Takano,
Tsuyoshi Inoue, Yusuke Mori, Soichi Owa & Takatomo Sasaki
Jpn. J. Appl. Phys. (2005) 44, L54-L56.
(58) Protein Crystallization by Combining Laser Irradiation and Solution Stirring Techniques
Hiroaki Adachi, Ai Niino, Satoshi Murakami, Kazufumi Takano, Hiroyoshi Matsumura,
Takayoshi Kinoshita, Masaichi Warizaya, Tsuyoshi Inoue, Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2005) 44, 1365-1366.
(59) Protein Crystal Growth Using Laser-Processed Seed Crystals
Kengo Takeuchi, Hiroshi Kitano, Hiroaki Adachi, Yusuke Mori, Takatomo Sasaki, Hiroyoshi Matsumura,
Tsuyoshi Inoue, Satoshi Murakami, Masaaki Doi, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Jpn. J. Appl. Phys. (2005) 44, 3177-3179.
(60) Crystallization and Preliminary X-ray Diffraction Study of Thermostable RNase HIII from Bacillus
stearothermophilus
Hyongi Chon, Hiroyoshi Matsumura, Yuiichi Koga, Kazufumi Takano & Shigenori Kanaya
Acta Cryst. (2005) F61, 293-295.
(61) Overproduction and Preliminary Crystallographic Study of a Human Kynurenine Aminotransferase II
Homologue from Pyrococcus horikoshii OT3
Hyongi Chon, Hiroyoshi Matsumura, Shoko Shimizu, Nao Maeda, Yuiichi Koga, Kazufumi Takano &
Shigenori Kanaya
Acta Cryst. (2005) F61, 319-321.
(62) Temperature-Screening System for Determining Protein Crystallization Conditions
Hiroaki Adachi, Ai Niino, Kazufumi Takano, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue,
Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2005) 44, 4080-4083.
(R11) 蛋白質の安定性とそこに秘めるもの
高野和文
生産と技術 (2005) 57, 43-45.
(63) Processing of Membrane Protein Crystal Using UV Laser Irradiation
Hiroshi Kitano, Satoshi Murakami, Hiroaki Adachi, Hiroyoshi Matsumura, Kazufumi Takano,
Tsuyoshi Inoue, Yusuke Mori, Soichi Owa & Takatomo Sasaki
J. Biosci. Bioeng. (2005) 100, 50-53.
(64) Gene Cloning and in vivo Characterization of a Dibenzothiophene Dioxygenase from Xanthobacter
polyaromaticivorans
Shin-ichi Hirano, Mitsuru Haruki, Kazufumi Takano, Tadayuki Imanaka, Masaaki Morikawa &
Shigenori Kanaya
Appl. Microbiol. Biotechnol. (2006) 69, 672-681.
(65) Crystal Structure of a Human Kynurenine Aminotransferase II Homologue from Pyrococcus horikoshii OT3
at 2.20 A Resolution
Hyongi Chon, Hiroyoshi Matsumura, Yuiichi Koga, Kazufumi Takano & Shigenori Kanaya
Proteins (2005) 61, 685-688.
(66) Solution Stirring Initiates Nucleation and Improves the Quality of Adenosine Deaminase Crystals
Hiroaki Adachi, Kazufumi Takano, Ai Niino, Hiroyoshi Matsumura, Takayoshi Kinoshita,
Masaichi Warizaya, Tsuyoshi Inoue, Yusuke Mori & Takatomo Sasaki
Acta Cryst. (2005) D61, 759-762.
(67) Semiautomatic Protein Crystallization System Featuring Crystallization Solution Preparation Function
Hiroaki Adachi, Hiroyoshi Matsumura, Tsuyoshi Inoue, Ai Niino, Kazufumi Takano, Satoshi Murakami,
Yusuke Mori & Takatomo Sasaki
Jpn. J. Appl. Phys. (2005) 44, 6302-6303.
(68) Femtosecond Laser Processing of Protein Crystals in Crystallization Drop
Masafumi Kashii, Hiroshi Kitano, Yoichiroh Hosokawa, Hiroaki Adachi, Yusuke Mori, Takatomo Sasaki,
Hiroshi Masuhara, Kazufumi Takano, Hiroyoshi Matsumura, Tsuyoshi Inoue, Satoshi Murakami,
Kazuomi Sugamoto & Hideki Yoshikawa
Jpn. J. Appl. Phys. (2005) 44, L873-L875.
(69) Structural Basis of Compound Recognition by Adenosine Deaminase
Takayoshi Kinoshita, Isao Nakanishi, Tadashi Terasaka, Masako Kuno, Nobuo Seki, Masaichi Warizaya,
Hiroyoshi Matsumura, Tsuyoshi Inoue, Kazufumi Takano, Hiroaki Adachi, Yusuke Mori & Takashi Fujii
Biochemistry (2005) 44, 10562-10569.
(70) Importance of a Repetitive Nine-residue Sequence Motif for Intracellular Stability and Functional Structure
of a Family I.3 Lipase
Clement Angkawidjaja, Aditya Paul, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
FEBS Let. (2005) 579, 4707-4712.
(71) Effect of Laser Irradiation on Enzyme Activity
Satoshi Murakami, Masafumi Kashii, Hiroshi Kitano, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura,
Tsuyoshi Inoue, Yusuke Mori, Masaaki Doi, Kazuomi Sugamoto, Hideki Yoshikawa & Takatomo Sasaki
Jpn. J. Appl. Phys. (2005) 44, 8216-8218.
(72) Phylogenetic Analysis of Condensation Domain in the Nonribosomal Peptide Synthetases
Niran Roongsawang, Siew Ping Lim, Kenji Washio, Kazufumi Takano, Shigenori Kanaya & Masaaki Morikawa
FEMS Microbiol. Lett. (2005) 252, 143-151.
(73) Binding Analysis of a Psychrotrophic FKBP22 to a Folding Intermediate of Protein Using Surface
Plasmon Resonance
Yutaka Suzuki, Ohnmar Ye Win, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
FEBS Let. (2005) 579, 5781-5784.
2006
(74) Amyloidogenecity and Pitrilysin Sensitivity of a Lysine-free Derivative of Amyloid β-peptide Cleaved
from a Recombinant Fusion Protein
Joel Cornista, Yuichi Koga, Kazufumi Takano, & Shigenori Kanaya
J. Biotechnol. (2006) 122, 186-197.
(75) Solution-Stirring Method Improves Crystal Quality of Human Triosephosphate Isomerase
Hiroaki Adachi, Ai Niino, Takayoshi Kinoshita, Masaichi Warizaya, Riyo Maruki, Kazufumi Takano,
Hiroyoshi Matsumura, Tsuyoshi Inoue, Satoshi Murakami, Yusuke Mori & Takatomo Sasaki
J. Biosci. Bioeng. (2006) 101, 83-86.
(76) Structure of Amyloid β Fragments in Aqueous Environments
Kazufumi Takano, Shuji Endo, Atsushi Mukaiyama, Hyongi Chon, Hiroyoshi Matsumura, Yuichi Koga
& Shigenori Kanaya
FEBS J. (2006) 273, 150-158.
(77) Crystal Structure and Structure-based Mutational Analyses of RNase HIII from Bacillus stearothermophilus:
a New Type 2 RNase H with TBP-like Substrate-binding Domain at the N-terminus
Hyongi Chon, Hiroyoshi Matsumura, Yuiichi Koga, Kazufumi Takano & Shigenori Kanaya
J. Mol. Biol. (2006) 356, 165-178.
(78) Effect of Ultrasonic Irradiation on Protein Crystallization
Keisuke Kakinouchi, Hiroaki Adachi, Hiroyoshi Matsumura, Tsuyoshi Inoue, Satoshi Murakami, Yusuke Mori,
Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
J. Cryst. Growth (2006) 292, 437-440.
(79) Cooling-rate Screening System for Determining Protein Crystal Growth Conditions
Ryota Murai, Shinya Nakata, Masafumi Kashii, Hiroaki Adachi, Ai Niino, Kazufumi Takano, Hiroyoshi Matsumura,
Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori & Takatomo Sasaki
J. Cryst. Growth (2006) 292, 433-436.
(80) Crystallization and Preliminary X-ray Analysis of the tRNA Thiolation Enzyme MnmA from Escherichia coli
complexed with tRNAGlu
Tomoyuki Numata, Y. Ikeuchi, Shuya Fukai, Hiroaki Adachi, Hiroyoshi Matsumura, Kazufumi Takano,
Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Takatomo Sasaki, T. Suzuki & Osamu Nureki
Acta Cryst. (2006) F62, 368-371.
(81) Purification, Crystallization and Preliminary X-ray Diffraction of SecDF, a Translocon-Associated
Membrane Protein, from Thermus thermophilus
Tomoya Tsukazaki, Hiroyuki Mori, Shuya Fukai, Tomoyuki Numata, Anna Perederina, Hiroaki Adachi,
Hiroyoshi Matsumura, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Takatomo Sasaki,
Dmitry G. Vassylyev, Osamu Nureki & Koreaki Ito
Acta Cryst. (2006) F62, 376-380.
(82) Identification of RNase HII from Psychrotrophic Bacterium, Shewanella sp. SIB1 as a High-activity Type RNase H
Hyongi Chon, Takashi Tadokoro, Naoto Ohtani, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
FEBS J. (2006) 273, 2264-2275.
(83) Ca2+-Dependent Maturation of Tk-subtilisin from a Hyperthermophilic Archaeon: Propeptide is a Potent Inhibitor of
the Mature Domain but is not Required for Its Folding
TMarian Pulido, Kenji Saito, Shun-ichi Tanaka, Yuichi Koga, Masaaki Morikawa, Kazufumi Takano & Shigenori Kanaya
Appl. Environ. Microbiol. (2006) 72, 4154-4162.
(84) Extracellular Secretion of E. coli Alkaline Phosphatase with a C-terminal Tag by Type I Secretion System:
Purification and Biochemical Characterization
Clement Angkawidjaja, Katsumasa Kuwahara, Kenji Omori, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Protein Eng. Des. Sel. (2006) 19, 337-343.
(R12) フェムト秒レーザー照射による結晶核発生と溶液攪拌による新しいタンパク質結晶化技術
森勇介、安達宏昭、高野和文、井上豪、村上聡、松村浩由、佐々木孝友
OPTRONICS (2006) 25, 201-207.
(85) Crystallization and Preliminary Crystallographic Analysis of Orotidine 5'-monophosphate Decarboxylase from the Human
Malaria Parasite Plasmodium falciparum
Sudaratana R. Krungkrai, Keiji Tokuoka, Yukiko Kusakari, Tsuyoshi Inoue, Hiroaki Adachi, Hiroyoshi Matsumura,
Kazufumi Takano, Satoshi Murakami, Yusuke Mori, Yasushi Kai, Jerapan Krungkrai & Toshihiro Horii
Acta Cryst. (2006) F62, 542-545.
(86) Biofilm Formation by Bacillus subtilis that Produces Gamma-Polyglutamate
Masaaki Morikawa, Shinji Kagihiro, Mitsuru Haruki, Kazufumi Takano, Steve Branda, Roberto Kolter & Shigenori Kanaya
Microbiology-SGM (2006) 152, 2801-2807.
(87) Crystallization and Preliminary Crystallographic Analysis of Type 1 RNase H from Hyperthermophilic Archaeon
Sulfolobus tokodaii 7
Dong-Ju You, Hyongi Chon, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Acta Cryst. (2006) F62, 781-784.
(R13) 創晶プロジェクトとバイオグリッドの連携による創薬開発の試み
井上豪、門祐示、有竹浩介、松村浩由、甲斐泰、福西快文、中村春木、木下誉富、仲西功、南方聖司、小松満男、
安達宏昭、森勇介、高野和文、村上聡、佐々木孝友、宮野雅司、裏出良博、坂田恒昭
SAR News (2006) 10, 2-6.
(88) Crystallization and Preliminary X-ray Diffraction Study of Active-site Mutant of Pro-Tk-subtilisin from a
Hyperthermophilic Archaeon
Shun-ichi Tanaka, Kenji Saito, Hyongi Chon, Hiroyoshi Matsumura, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Acta Cryst. (2006) F62, 902-905.
(89) Application of Femtosecond Laser Ablation for Detaching Grown Protein Crystals from a Glass Capillary Tube
Masafumi Kashii, Ryo Fujisawa, Hiroaki Adachi, Yusuke Mori, Takatomo Sasaki, Yuichi Koga, Kazufumi Takano,
Shigenori Kanaya, Hiroyoshi Matsumura, Tsuyoshi Inoue, Satoshi Murakami, Kazuomi Sugamoto & Hideki Yoshikawa
J. Biosci. Bioeng. (2006) 102, 372-374.
(R14) タンパク質・有機分子の新しい結晶化技術
安達宏昭、高野和文、森勇介
ファルマシア (2006) 24, 1237-1240.
(90) A Hyperthermophilic Protein Acquires Function at the Cost of Stability
Atsushi Mukaiyama, Mitsuru Haruki, Motonori Ota, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Biochemistry (2006) 45, 12673-12679.
(R15) フェムト秒レーザー照射によるタンパク質・有機低分子の結晶化
安達宏昭、森勇介、佐々木孝友、高野和文、松村浩由、井上豪、村上聡
酵素工学ニュース (2006) 56, 9-13.
(R16) 低温菌由来タンパク質の利用
古賀雄一、板東敬祐、高野和文、金谷茂則
実用産業情報 (2006) 39, 20-23.
(R17) 枯草菌バイオフィルム形成遺伝子の探索
森川正章、河合良尚、相原悠、鷲尾健司、高野和文、金谷茂則
生物工学会誌 (2006) 84, 488-490.
2007
(91) Gentisate 1,2-dioxygenase from Xanthobacter Polyaromaticivorans 127W
Shin-ichi Hirano, Masaaki Morikawa, Kazufumi Takano, Tadayuki Imanaka & Shigenori Kanaya
Biosci. Biotechnol. Biochem. (2007) 71, 192-199.
(92) Femtosecond Laser-induced Cleaving of Protein Crystal in Water Solution
Masafuki Kashii, Yoichiroh Hosokawa, Hiroshi Kitano, Hiroaki Adachi, Yusuke Mori, Kazufumi Takano,
Hiroyoshi Matsumura, Tsuyoshi Inoue, Satoshi Murakami, Kazuomi Sugamoto, Hideki Yoshikawa, Takatomo Sasaki &
Hiroshi Masuhara
Appl. Sur. Sci. (2007) 253, 6447-6450.
(P2) Development of Protein Crystallization and Processing: Femtosecond Laser, All Solid-state 193 nm Laser, and
Solution Stirring Techniques
Yusuke Mori, Kazufumi Takano, Hiroaki Adachi, Tsuyoshi Inoue, Satoshi Murakami, Hiroyoshi Matsumura,
Masafuki Kashii, Hiroshi Y. Yoshikawa, Syou Maki, Tomoya Kitatani, Shino Okada & Takatomo Sasaki
Proceedings of SPIE (2007) 6460, 646008.
(93) Crystallization and Preliminary X-ray Diffraction Study of Glycerol Kinase from Hyperthermophilic Archaeon
Thermococcus kodakaraensis
Ryota Katsumi, Yuichi Koga, Dong-Ju You, Hiroyoshi Matsumura, Kazufumi Takano & Shigenori Kanaya
Acta Cryst. (2007) F63, 126-129.
(94) Directed Evolution of Tk-subtilisin from Hyperthermophilic Archaeon: Identification of Single Amino Acid
Substitution Responsible for Low-temperature Adaptation
Marian Pulido, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Protein Eng. Des. Sel. (2007) 20, 143-153.
(95) Crystal Structure of Unautoprocessed Precursor of Subtilisin from a Hyperthermophilic Archaeon: Evidence
for Ca2+-induced Folding
Shun-ichi Tanaka, Kenji Saito, Hyongi Chon, Hiroyoshi Matsumura, Yuichi Koga, Kazufumi Takano &
Shigenori Kanaya
J. Biol. Chem. (2007) 282, 7825-7832.
(R18) タンパク質光結晶化技術
安達宏昭、森勇介、佐々木孝友、高野和文、井上豪、松村浩由、村上聡
光学 (2007) 36, 10-14.
(96) Extracellular Overproduction and Preliminary Crystallographic Analysis of a Family I.3 Lipase
Clement Angkawidjaja, Dong-Ju You, Hiroyoshi Matsumura, Yuichi Koga, Kazufumi Takano &
Shigenori Kanaya
Acta Cryst. (2007) F63, 187-189.
(97) Conformational Contagion in a Protein: Structural Properties of a Chameleon Sequence
Kazufumi Takano, Yoshiaki Katagiri, Atsushi Mukaiyama, Hyongi Chon, Hiroyoshi Matsumura, Yuichi
Koga & Shigenori Kanaya
Proteins (2007) 68, 617-625.
(B2) フェムト秒レーザー照射による結晶核発生と溶液撹拌による新しいタンパク質結晶化技術
森勇介、安達宏昭、高野和文、井上豪、村上聡、松村浩由、佐々木孝友
バイオプロセスハンドブック、エヌティーエス出版 (2007) 340-347.
(98) Structural, Thermodynamic, and Mutational Analyses of a Psychrotrophic RNase HI
Takashi Tadokoro, Dong-Ju You, Yumi Abe, Hyongi Chon, Hiroyoshi Matsumura, Yuichi Koga,
Kazufumi Takano & Shigenori Kanaya
Biochemistry (2007) 46, 7460-7468.
(99) Identification of the Gene Encoding a Type 1 RNase H with an N-terminal Double-Stranded RNA Binding
Domain from a Psychrotrophic Bacterium
Takashi Tadokoro, Hyongi Chon, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
FEBS J. (2007) 274, 3715-3727.
(P3) Drug Development Value Chain Constructed by Collaboration Between The SOSHO Project and The NPO BIOGRID
Tsuyoshi Inoue, Yuji Kado, Keiji Tokuoka, Hiroyoshi Matsumura, Yasushi Kai, Yusuke Mori,
Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Yoshifumi Fukunishi, Haruki Nakamura,
Takayoshi Kinoshita, Isao Nakanishi, Yasushi Okuno, Satoshi Minakata & Tsuneaki Sakata
AIP Conference Proceedings (2007) 902, 85-88.
(100) Four New Crystal Structures of Tk-subtilisin in Unautoprocessed, Autoprocessed and Mature Forms:
Insight in Structural Changes during Maturation Process
Shun-ichi Tanaka, Hiroyoshi Matsumura, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
J. Mol. Biol. (2007) 372, 1055-1069.
(101) Protein Thermostabilization Requires a Fine-tuned Placement of Surface Charged Residues
Dong-Ju You, Satoshi Fukuchi, Ken Nishikawa, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
J. Biochem. (2007) 142, 507-516.
(102) Crystal Structure of Type 1 RNase H from Hyperthermophilic Archaeon Sulfolobus tokodaii: Role of
Arg118 and C-terminal Anchoring
Dong-Ju You, Hyongi Chon, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Biochemistry (2007) 46, 11494-11503.
(103) Structural and Thermodynamic Analyses of Escherichia coli Ribonuclease HI Variant with
Quintuple Thermostabilizing Mutations
Mitsuru Haruki, Masaki Tanaka, Takehiko Motegi, Takashi Tadokoro, Yuichi Koga, Kazufumi Takano &
Shigenori Kanaya
FEBS J. (2007) 274, 5815-5825.
(104) Crystal Structure of a Family I.3 Lipase from Pseudomonas sp. MIS38 in a Closed Conformation
Clement Angkawidjaja, Dong-Ju You, Hiroyoshi Matsumura, Katsumasa Kuwahara, Yuichi Koga,
Kazufumi Takano & Shigenori Kanaya
FEBS Let. (2007) 581, 5060-5064.
(105) Requirement of Left-handed Glycine Residue for High Stability of the Tk-subtilisin Propeptide as
Revealed by Mutational and Crystallographic Analyses
Marian Pulido, Shun-ichi Tanaka, Chutima Sringiew, Dong-Ju You, Hiroyoshi Matsumura, Yuichi Koga,
Kazufumi Takano & Shigenori Kanaya
J. Mol. Biol. (2007) 374, 1359-1373.
(R19) フェムト秒レーザーによるタンパク質結晶化の制御
吉川洋史、村井良多、牧祥、北谷友也、杉山成、安達宏昭、高野和文、松村浩由、村上聡、井上豪、佐々木孝友、森勇介
No.RTM-07-40 (2007) 7-11.
(R20) 創薬バリューチェインによるIn silico創薬研究の紹介
井上豪、安達宏昭、森勇介、高野和文、松村浩由、村上聡、福西快文、中村春木、木下誉富、仲西功、奥野恭史
南方聖司、佐久間俊広、高田俊和、北島正人、福岡良忠、坂田恒昭
CBI Monograph Series 2007 (2007) 81-85.
2008
(106) Osmolyte Effect on the Stability and Folding of a Hyperthermophilic Protein
Atsushi Mukaiyama, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Proteins (2008) 71, 110-118.
(107) Effect of Solution Flow Produced by Rotary Shaker on Protein Crystallization
Ryota Murai, Hiroshi Y. Yoshikawa, Hisato Kawahara, Syou Maki, Shigeru Sugiyama, Tomoya Kitatani, Hiroaki
Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Takatomo Sasaki & Yusuke Mori
J. Cryst. Growth (2008) 310, 2168-2172.
(108) Protein Crystallization in a 100 nl Solution with New Stirring Equipment
Syou Maki, Ryota Murai, Hiroshi Y. Yoshikawa, Tomoya Kitatani, Shinya Nakata, Hisato Kawahara, Hitoshi Hasenaka,
Asako Kobayashi, Shino Okada, Shigeru Sugiyama, Hiroaki Adachi, Hiroyoshi Matsumura, Kazufumi Takano,
Satoshi Murakami, Tsuyoshi Inoue, Takatomo Sasaki & Yusuke Mori
J. Synchrotron Rad. (2008) 15, 269-272.
(109) New Technique of Manipulating a Protein Crystal Using Adhesive Material
Tomoya Kitatani, Shigeru Sugiyama, Hiroyoshi Matsumura, Hiroaki Adachi, Hiroshi Y. Yoshikawa, Syou Maki,
Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori & Kazufumi Takano
Appl. Phys. Express (2008) 1, 037002.
(110) Hydrophobic Effect on the Stability and Folding of a Hyperthermophilic Protein
Hongju Dong, Atsushi Mukaiyama, Takashi Tadokoro, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
J. Mol. Biol. (2008) 378, 264-272.
(P4) Crystal Structure of Amyloid Beta Fragment
Kazufumi Takano, Yoshiki Takeda, Dong-Ju You, Atsushi Mukaiyama, Hiroyoshi Matsumura, Yuichi Koga &
Shigenori Kanaya
New Trends in Alzheimer and Parkinson Related Disorders (2008) 153-156.
(111) Crystal Structure of Highly Thermostable Glycerol Kinase from a Hyperthermophilic Archaeon in a Dimeric Form
Yuichi Koga, Ryota Katsumi, Dong-Ju You, Hiroyoshi Matsumura, Kazufumi Takano & Shigenori Kanaya
FEBS J. (2008) 275, 2632-2643.
(R21) 膜タンパク質の結晶化技術の新展開及び創薬バリューチェインの紹介
井上豪、安達宏昭、村上聡、高野和文、松村浩由、森勇介、福西快文、中村春木、木下誉富、仲西功、奥野恭史
南方聖司、下条真司、坂田恒昭
薬学雑誌 (2008) 128, 497-505.
(R22) Amyloid β Conformation in Aqueous Environment
Kazufumi Takano
Curr. Alzheimer Res. (2008) 5, 540-547.
(R23) フェムト秒レーザー誘起タンパク質結晶核発生
森勇介、安達宏昭、高野和文、井上豪、村上聡、松村浩由、吉川洋史
O plus E (2008) 30, 480-484.
(BE1) タンパク質結晶の新展開〜新しい育成技術から構造解析・応用研究へ〜
高野和文（監修）
シーエムシー出版 (2008)
(B3) 創薬バリューチェインによるIn silico創薬に関する研究
井上豪、福西快文、中村春木、木下誉富、仲西功、奥野恭史、南方聖司、佐久間俊広、高田俊和、北島正人、
福岡良忠、奥村利幸、安達宏昭、森勇介、高野和文、松村浩由、村上聡、坂田恒昭
タンパク質結晶の新展開〜新しい育成技術から構造解析・応用研究へ〜、シーエムシー出版 (2008) 230-239.
(112) Remarkable Stabilization of a Psychrotrophic RNase HI by Combination of Thermostabilizing Mutations
Identified by Suppressor Mutation Method
Takashi Tadokoro, Kyoko Matsushita, Yumi Abe, Muhammad Saifur Rohman, Yuichi Koga, Kazufumi Takano
& Shigenori Kanaya
Biochemistry (2008) 47, 8040-8047.
(113) Laser Energy Dependence on Femtosecond Laser-induced Nucleation of Protein
Hiroshi Y. Yoshikawa, Ryota Murai, Syou Maki, Tomoya Kitatani, Shigeru Sugiyama, Gen Sazaki, Hiroaki Adachi,
Tsuyoshi Inoue, Hiroyoshi Matsumura, Kazufumi Takano, Satoshi Murakami, Takatomo Sasaki & Yusuke Mori
Appl. Phys. (2008) A93, 911-915.
(MP1) 熱安定性解析：熱量測定
向山厚、安東菜津美、高野和文
蛋白質科学会アーカイブ (2008) 1, e039.
(R24) フェムト秒レーザー核発生と溶液撹拌による新しいタンパク質結晶化技術
森勇介、安達宏昭、高野和文、井上豪、村上聡、松村浩由
ケミカルエンジニヤリング (2008) 53, 22-27.
(114) Effect of the Disease-causing Mutations Identified in Human RNase H2 on the Activities and Stabilities of
Yeast RNase H2 and Archaeal RNase HII
Muhammad S. Rohman, Yuichi Koga, Kazufumi Takano, Hyongi Chon, Robert J. Crouch & Shigenori Kanaya
FEBS J. (2008) 275, 4836-4849.
(R25) 短パルスレーザーによるタンパク質結晶加工
吉川洋史、村井良多、北谷友也、安達宏昭、高野和文、松村浩由、井上豪、村上聡、佐々木孝友、森勇介
オプトロニクス (2008) 27, 153-161.
(115) Crystallization and Preliminary X-ray Diffraction Studies of an RNA Aptamer in Complex with the
Human IgG Fc Fragment
Shigeru Sugiyama, Yusuke Nomura, Taiichi Sakamoto, Tomoya Kitatani, Asako Kobayashi, Shin Miyakawa,
Yoshinori Takahashi,Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori,
Yoshikazu Nakamura & Hiroyoshi Matsumura
Acta Cryst. (2008) F64, 942-944.
(116) Crystallization and Preliminary Neutron Diffraction Studies of HIV-1 Protease Cocrystallized with Inhibitor KNI-272
Hiroyoshi Matsumura, Motoyasu Adachi, Shigeru Sugiyama, Shino Okada, Megumi Yamakami, Taro Tamada, Koushi
Hidaka, Yoshio Hayashi, Tooru Kimura, Yoshiaki Kiso, Tomoya Kitatani, Sho Maki, Hiroshi Y. Yoshikawa,
Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Ryota Kuroki & Yusuke Mori
Acta Cryst. (2008) F64, 1003-1006.
(117) Evaluation and Improvement of a Technique to Manipulate Protein Crystals in Solution
Tomoya Kitatani, Hiroaki Adachi, Shigeru Sugiyama, Hiroyoshi Matsumura, Ryota Murai, Yoshinori Takahashi,
Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori & Kazufumi Takano
Jpn. J. Appl. Phys. (2008) 47, 8995-8997.
(R26) 超好熱菌由来蛋白質の熱力学的安定化機構
向山厚、高野和文
熱測定 (2008) 35, 237-243.
(118) Importance of the Ca2+-binding Sites in the N-catalytic Domain of a Family I.3 Lipase for Activity and Stability
Katsumasa Kuwahara, Clement Angkawidjaja, Hiroyoshi Matsumura, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Protein Eng. Des. Sel. (2008) 21, 737-744.
(119) Crystal Structure of Tk-subtilisin Folded without Propeptide: Requirement of Propeptide for Acceleration of Folding
Shun-ichi Tanaka, Yuki Takeuchi, Hiroyoshi Matsumura, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
FEBS Let. (2008) 582, 3875-3878.
(120) Crystallization and Preliminary X-ray Crystallographic Analysis of Ca2+-free Primary Ca2+-sensor of Na+/Ca2+ Exchanger
Masashi Mima, Chika Kawai, Kumsun Paku, Koji Tomoo, Toshimasa Ishida, Shigeru Sugiyama, Hiroyoshi Matsumura,
Tomoya Kitatani, Hiroshi Y. Yoshikawa, Syou Maki, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami,
Tsuyoshi Inoue, Yusuke Mori, Satomi Kita & Takahiro Iwamoto
Acta Cryst. (2008) F64, 1125-1127.
(R27) Protein Crystallization Using Femtosecond Laser Irradiation and Solution Stirring
Kazufumi Takano, Hiroshi Y. Yoshikawa, Tomoya Kitatani, Shigeru Sugiyama, Yoshinori Takahashi, Ryota Murai,
Syou Maki, Hiroaki Adachi, Hiroyoshi Matsumura, Tsuyoshi Inoue, Satoshi Murakami & Yusuke Mori
Curr. Topics in Peptide & Protein Res. (2008) 9, 1-9.
2009
(121) Femtosecond Laser-Induced Nucleation of Protein in Agarose Gel
Hiroshi Y. Yoshikawa, Ryota Murai, Shigeru Sugiyama, Gen Sazaki, Tomoya Kitatani, Yoshinori Takahashi,
Hiroaki Adachi, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Kazufumi Takano & Yusuke Mori
J. Cryst. Growth (2009) 311, 956-959.
(122) Proline Effect on the Thermostability and Slow Unfolding of a Hyperthermophilic Protein
Kazufumi Takano, Ryogo Higashi, Jun Okada, Atsushi Mukaiyama, Takashi Tadokoro, Yuichi Koga & Shigenori Kanaya
J. Biochem. (2009) 145, 79-85.
(123) Destabilization of Psychrotrophic RNase HI in a Localized Fashion as Revealed by Mutational and X-ray Crystallographic Analyses
Muhammad S. Rohman, Takashi Tadokoro, Clement Angkawidjaja, Yumi Abe, Hiroyoshi Matsumura, Yuichi Koga,
Kazufumi Takano & Shigenori Kanaya
FEBS J. (2009) 276, 603-613.
(124) Structure of HIV-1 Protease in Complex with Potent Inhibitor KNI-272 Determined by High Resolution X-ray and
Neutron Crystallography
Motoyasu Adachi, Takashi Ohhara, Kazuo Kurihara, Taro Tamada, Eijiro Honjo, Nobuo Okazaki, Shigeki Arai,
Yoshinari Shoyama, Kaname Kimura, Hiroyoshi Matsumura, Shigeru Sugiyama, Hiroaki Adachi, Kazufumi Takano,
Yusuke Mori, Koushi Hidaka, Tooru Kimura, Yoshio Hayashi, Yoshiaki Kiso & Ryota Kuroki
Proc. Natl. Acad. Sci. USA (2009) 106, 4641-4646.
(R28) Slow Unfolding of Monomeric Proteins from Hyperthermophiles with Reversible Unfolding
Atsushi Mukaiyama & Kazufumi Takano
Int. J. Mol. Sci. (2009) 10, 1369-1385.
(125) Protein Crystallization in Agarose Gel with High Strength: Developing an Automated System for Protein Crystallographic Processes
Shigeru Sugiyama, Kana Tanabe, Mika Hirose, Tomoya Kitatani, Hiroshi Hasenaka, Yoshinori Takahashi, Hiroaki Adachi,
Kazufumi Takano, Satoshi Murakami, Yusuke Mori, Tsuyoshi Inoue & Hiroyoshi Matsumura
Jpn. J. Appl. Phys. (2009) 48, 075502.
(126) Engineering of Monomeric FKBP22 with Ppiase Activity: Importance of a V-Shaped Dimeric Structure for Binding to
Protein Substrate
Cahyo Budiman, Keisuke Bando, Clement Angkawidjaja, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
FEBS J. (2009) 276, 4091-4101.
(B4) Ｘ線構造解析による検証
安達宏昭、高野和文、松村浩由、村上聡、井上豪、森勇介
コンピューターで薬を創ろう、ケイ・ディー・ネオブック (2009) 71-80.
(127) Femtosecond Laser Processing of Agarose Gel Surrounding Protein Crystals for the Development of an Automated Crystal
Capturing System
Shigeru Sugiyama, Hiroshi Hasenaka, Mika Hirose, Noriko Shimizu, Tomoya Kitatani, Yoshinori Takahashi, Hiroaki Adachi,
Kazufumi Takano, Satoshi Murakami, Yusuke Mori, Tsuyoshi Inoue & Hiroyoshi Matsumura
Jpn. J. Appl. Phys. (2009) 48, 105502.
(128) A Manipulating Tool for Protein Microcrystals in Solution using Adhesive Materials
Tomoya Kitatani, Hiroaki Adachi, Shigeru Sugiyama, Hiroyoshi Matsumura, Ryota Murai, Yoshinori Takahashi,
Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori & Kazufumi Takano
Jpn. J. Appl. Phys. (2009) 48, 118001.
(129) Identification of the Interactions Critical for Propeptide-Catalyzed Folding of Tk-subtilisin
Shun-ichi Tanaka, Hiroyoshi Matsumura, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
J. Mol. Biol. (2009) 394, 306-319.
(130) Requirement of a Unique Ca2+-Binding Loop for Folding of Tk-subtilisin from a Hyperthermophilic Archaeon
Yuki Takeuchi, Shun-ichi Tanaka, Hiroyoshi Matsumura, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Biochemistry (2009) 48, 10637-10643.
(131) Growth of Large Protein Crystals by Top-seeded Solution Growth Together with Floating and Solution-stirring Technique
Noriko Shimizu, Shigeru Sugiyama, Mihoko Maruyama, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Hiroaki Adachi,
Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Hiroyoshi Matsumura & Yusuke Mori
Cryst. Growth Des. (2009) 9, 5227-5232.
(132) Promotion of Crystal Nucleation of Protein by Semi-solid Agarose Gel
Kana Tanabe, Mika Hirose, Ryota Murai, Shigeru Sugiyama, Noriko Shimizu, Mihoko Maruyama, Yoshinori Takahashi, Hiroaki
Adachi, Kazufumi Takano, Satoshi Murakami, Yusuke Mori, Eiichi Mizohata, Tsuyoshi Inoue & Hiroyoshi Matsumura
Appl. Phys. Express (2009) 2, 125501.
(133) Femtosecond Laser Processing of Protein Crystals Grown in Agarose Gel
Hiroshi Hasenaka, Shigeru Sugiyama, Mika Hirose, Noriko Shimizu, Tomoya Kitatani, Yoshinori Takahashi, Hiroaki Adachi,
Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori & Hiroyoshi Matsumura
J. Cryst. Growth (2009) 312, 73-78.
2010
(R29) 創薬バリューチェインのインシリコ技術を活用した阻害剤開発の試み
井上豪、安達宏昭、森勇介、高野和文、松村浩由、村上聡、福西快文、中村春木、木下誉富、仲西功、奥野恭史
南方聖司、佐久間俊広、高田俊和、北島正人、福岡良忠、坂田恒昭
日本結晶学会誌 (2010) 52, 89-94.
(134) Subtilisin-like Serine Protease from Hyperthermophilic Archaeon Thermococcus kodakaraensis with N- and C-terminal Propeptides
Tita Foophow, Shun-ichi Tanaka, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Protein Eng. Des. Sel. (2010) 23, 347-355.
(135) Enhancement of Femtosecond Laser-induced Nucleation of Protein in a Gel Solution
Ryota Murai, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Mihoko Maruyama, Shigeru Sugiyama, Gen Sazaki, Hiroaki Adachi,
Kazufumi Takano, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue & Yusuke Mori
Appl. Phys. Let. (2010) 96, 043702.
(136) Urea Denatured State Ensembles Contain Extensive Secondary Structure that is Increased in Hydrophobic Proteins
C. Nick Pace, Beatrice M. P. Huyghues-Despointes, Hailong Fu, Kazufumi Takano, J. Martin Scholtz &
Gerald R. Grimsley
Protein Sci. (2010) 19, 929-943.
(137) Cloning of the RNase H Genes from a Metagenomic DNA Library: Identification of a New Type 1 RNase H
without a Typical Active-Site Motif
Eiko Kanaya, Tomoko Sakabe, Nhan Nguyen, Satoshi Koikeda, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
J. Appl. Microbiol. (2010) 109, 974-983.
(138) Protein Core Adaptability: Crystal Structures of the Cavity-Filling Variants of Escherichia coli RNase HI
Masaki Tanaka, Hyongi Chon, Clement Angkawidjaja, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Protein Pept. Lett. (2010) 17, 1163-1169.
(139) Molecular resolution investigation of tetragonal lysozyme(110) face in liquid by FM-AFM
Ken Nagashima, Masayuki Abe, Seizo Morita, Noriaki Oyabu, Kei Kobayashi, Hirofumi Yamada, Masahiro Ohta, Ryohei Kokawa,
Ryota Murai, Hiroyoshi Matsumura, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue & Yusuke Mori
J. Vac. Sci. Technol. (2010) B28, C4C11-C4C14.
(140) X-ray Crystallographic and MD Simulation Studies on the Interfacial Activation Mechanism of a Family I.3 Lipase with Two Lids
Clement Angkawidjaja, Hiroyoshi Matsumura, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
J. Mol. Biol. (2010) 400, 82-95.
(141) Growth of Large Protein Crystals by a Large-Scale Hanging Drop Method
Keisuke Kakinouchi, Tsutomu Nakamura, Taro Tamada, Hiroaki Adachi, Shigeru Sugiyama, Mihoko Maruyama, Yoshinori Takahashi,
Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Ryota Kuroki, Yusuke Mori & Hiroyoshi Matsumura
J. Appl. Cryst. (2010) 43, 937-939.
(142) Crystal Growth Procedure of HIV-1 Protease-inhibitor KNI-272 Complex for Neutron Structural Analysis at 1.9A Resolution
Noriko Shimizu, Shigeru Sugiyama, Mihoko Maruyama, Yoshinori Takahashi, Motoyasu Adachi, Taro Tamada,
Koushi Hidaka, Yoshio Hayashi, Tooru Kimura, Yoshiaki Kiso, Hiroaki Adachi, Kazufumi Takano,
Satoshi Murakami, Tsuyoshi Inoue, Ryota Kuroki, Yusuke Mori & Hiroyoshi Matsumura
Cryst. Growth Des. (2010) 10, 2990-2994.
(143) Crystal Structure of a Subtilisin Homologue, Tk-SP, from Thermococcus kodakaraensis: Requirement of a C-terminal β-jelly
Roll Domain for Hyperstability
Tita Foophow, Shun-ichi Tanaka, Clement Angkawidjaja, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
J. Mol. Biol. (2010) 400, 865-877.
(144) Estimated Effects of Silicone Glue on Protein Crystal Growth
Mihoko Maruyama, Noriko Shimizu, Shigeru Sugiyama, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano,
Satoshi Murakami, Tsuyoshi Inoue, Hiroyoshi Matsumura & Yusuke Mori
J. Cryst. Growth (2010) 312, 2771-2774.
(145) Conformational Plasticity of RNA for Target Recognition as Revealed by the 2.15 A Crystal Structure of a Human IgG-Aptamer Complex
Yusuke Nomura, Shigeru Sugiyama, Taiichi Sakamoto, Shin Miyakawa, Hiroaki Adachi, Kazufumi Takano,
Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Yoshikazu Nakamura & Hiroyoshi Matsumura
Nucleic Acids Res. (2010) 38, 7822-7829.
(146) Evolution and Thermodynamics of Slow Unfolding of Hyperstable Monomeric Proteins
Jun Okada, Tomohiro Okamoto, Atsushi Mukaiyama, Takashi Tadokoro, Dong-Ju You, Hyongi Chon, Yuichi Koga,
Kazufumi Takano & Shigenori Kanaya
BMC Evol. Biol. (2010) 10, 207.
(147) Importance of an N-terminal Hybrid Binding Domain for Substrate Binding and Mg2+-dependent Activity of RNase HI from Thermotoga maritima
Nujarin Jongruja, Dong-Ju You, Eiko Kanaya, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
FEBS J. (2010) 277, 4474-4489.
(P5) Localization of the HOXC Homeobox Gene Family During Palate Formation in Mice
Azumi Hirata, Takehito Tsuji, Hideto Imura, Tomohiro Yamada, Takaaki Ueno, Katsuaki Mishima, Nagato Natsume,
Toshio Sugahara, Yuichi Koga, Kazufumi Takano & Hiroaki Nakamura
XX Congress of the EACMFS (2010) 415-420.
2011
(B5) Folding and Unfolding of Hyperthermophilic Proteins; Molecular Basis of Adaptation to Hot Environment
Atsushi Mukaiyama & Kazufumi Takano
Protein Folding (E. C. Walters, ed.) (2011) pp. 443-464, Nova Science, Hauppauge (NY).
(148) Flavobacterium Banpakuense sp. nov. Isolated from Leaf-and-branch Compost
Joong-Jae Kim, Hyun Mi Jin, Hyo Jung Lee, Che Ok Jeon, Eiko Kanaya, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Int. J. Syst. Evol. Microbiol. (2011) in press.
(149) Crystal Structure of Stable Protein, CutA1, from Psychrotrophic Bacterium Shewanella sp. SIB1
Aya Sato, Sonoko Yokotani, Takashi Tadokoro, Shun-ichi Tanaka, Clement Angkawidjaja, Yuichi Koga,
Kazufumi Takano & Shigenori Kanaya
J. Synchrotron Rad. (2011) 18, 6-10.
(150) Approach for Growth of High-Quality and Large Protein Crystals
Hiroyoshi Matsumura, Shigeru Sugiyama, Mika Hirose, Keisuke Kakinouchi, Mihoko Maruyama, Ryota Murai, Hiroaki Adachi,
Kazufumi Takano, Satoshi Murakami, Yusuke Mori & Tsuyoshi Inoue
J. Synchrotron Rad. (2011) 18, 16-19.
(151) Laser-induced Nucleation in Protein Crystallization: Local Increase in Protein Concentration Induced by Femtosecond Laser Irradiation
Natsuko Iefuji, Ryota Murai, Mihoko Maruyama, Yoshinori Takahashi, Shigeru Sugiyama, Hiroaki Adachi, Hiroyoshi Matsumura,
Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
J. Cryst. Growth (2011) 318, 741-744.
(152) Inhibition of Chymotrypsin- and Subtilisin-like Serine Proteases with Tk-serpin from Hyperthermophilic Archaeon
Thermococcus kodakaraensis
Shun-ichi Tanaka, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Biochim. Biophys. Acta (2011) 1814, 299-307.
(153) Effect of Evaporation on Protein Crystals Grown in Semi-solid Agarose Hydrogel
Shigeru Sugiyama, Mika Hirose, Noriko Shimizu, Mayumi Niiyama, Mihoko Maruyama, Gen Sazaki, Ryota Murai, Hiroaki Adachi,
Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori & Hiroyoshi Matsumura
Jpn. J. Appl. Phys. (2011) 50, 025502.
(154) Importance of an Extreme C-terminal Motif of a Family I.3 Lipase for Stability
Katsumasa Kuwahara, Clement Angkawidjaja, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
Protein Eng. Des. Sel. (2011) in press.
(155) Stabilization by Fusion to the C-terminus of Hyperthermophile Sulfolobus tokodaii RNase HI: A Possibility of Protein Stabilization Tag
Kazufumi Takano, Tomohiro Okamoto, Jun Okada, Shun-ichi Tanaka, Clement Angkawidjaja, Yuichi Koga & Shigenori Kanaya
PLoS ONE (2011) 6, e16226.
(R30) 結晶成長の新技術
松村浩由、杉山成、安達宏昭、高野和文、村上聡、井上豪、森勇介
バイオインダストリー (2011) 28, 30-36.
(B6) Delineation of the Conformational Thermostability of Hyperthermophilic Proteins Based on Structural and Biophysical Analyses
Atsushi Mukaiyama & Kazufumi Takano
Thermostable Proteins: Structural Stability and Design (S. Sen & L. Nilsson, eds.) (2012) pp. -, CRC Press, Boca Raton (FL).
(156) An Alternative Mature Form of Subtilisin Homologue, Tk-SP, from Thermococcus kodakaraensis Identified in the Presence of Ca2+
Nitat Sinsereekul, Tita Foophow, Mai Yamanouchi, Yuichi Koga, Kazufumi Takano & Shigenori Kanaya
FEBS J. (2011) in press.
(157) Growth of Protein Crystals by Syringe-Type Top-Seeded Solution Growth
Hiroyoshi Matsumura, Keisuke Kakinouchi, Tsutomu Nakamura, Shigeru Sugiyama, Mihoko Maruyama, Hiroaki Adachi,
Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue & Yusuke Mori
Cryst. Growth Des. (2011) in press.
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