Name: Shigenori Kanaya
Date of Birth: January 22, 1950
Place of Birth: Mie, Japan
Nationality: Japanese
Address (office): Department of
Material and Life Science
Graduate School of Engineering,
Osaka University
2-1, Yamadaoka, Suita, Osaka
565-0871, Japan
Tel/Fax. +81-6-6879-7938
E-mail.
kanaya@mls.eng.osaka-u.ac.jp
Education:
B.
Sc. Department of Chemistry, Faculty of Science, Tohoku University,
April, 1968 - March, 1973.
M.
Sc. Department of Chemistry, Faculty of Science, Tohoku University,
April, 1973 - March, 1975.
Ph.
D. Department of Chemistry, Faculty of Science, Tohoku University,
April, 1975 - March, 1979.
Thesis title: Active site of phosphomonoesterase-phosphodiesterase from Fusarium moniliforme.
Professional Experiences:
April
1979 - October 1980
Post doctoral fellow at Mitsubishi Kasei Institute of Life Sciences
Field of study: Purification and characterization of various secretory RNases.
October
1980 - June 1983
Post doctoral fellow at Laboratory of Molecular Genetics, NICHHD, NIH (USA)
Field of study: Cloning, sequencing, gene expression, and mutagenesis of E. coli RNase H1.
July
1983 - April 1986
Researcher at Research Center of Mitsubishi Kasei Corporation
Field of study: Purification and characterization of recombinant proteins
overproduced in E. coli.
May
1986 - May 1995
Senior researcher (May 1986 - March 1992), Senior scientist (April 1992 - March
1993), and Deputy manager (April 1993 - May 1995) at Protein Engineering
Research Institute
Field of study: Studies on the structures and functions of RNases H with
protein engineering technology.
June
1995 -
Professor at Department of Material and Life Science, Graduate School of
Engineering, Osaka University
Field of study: Studies on the structures and functions of proteins isolated
from microorganisms that grow under unusual environment.
Field of study:
1) Studies on the structures and functions of RNase H enzymes.
2) Establishment of the methods to control enzyme functions and protein
stability with protein engineering technology.
3) Studies on the structures and functions of proteins isolated from
microorganisms that grow under extremely unusual environment.
4)
Studies on the structures and functions of proteins isolated from leaf-branch
compost using metagenomic approach
List of publication (original
papers):
1. Fujimoto,
D., and Kanaya, S. (1973) Cuticlin: A noncollagen structural protein from Ascaris cuticle. Arch. Biochem. Biophys. 157,
1-6.
2. Kanaya,
S., and Fujimoto, D. (1973) Fragments of polyproline-like structure from a
structural protein, cuticlin. J. Mol.
Biol. 81, 415-418.
3. Kanaya,
S., and Yoshida, H. (1979) Phosphodiesterase-phosphomono-esterase from Fusarium moniliforme: separation and
properties of four isozymes. J. Biochem.
85, 791-797.
4. Kanaya,
S., and Uchida, T. (1981) Purification of ribonuclease T1 by
affinity chromatography. J. Biochem. 89, 591-597.
5. Kanaya,
S., and Uchida, T. (1981) An affinity adsorbent, 5'-adenylate-aminohexyl-sepharose.
II. Purification and characterization of multi-forms of RNase T2. J. Biochem. 90, 473-481.
6. Crouch,
R. J., Kanaya, S., and Earl, P. L. (1983) A model for the involvement of the
small nucleolar RNA (U3) in processing eukaryotic ribosomal RNA. Molec. Biol. Rep. 9, 75-78.
7. Kanaya,
S., and Crouch, R. J. (1983) DNA sequence of the gene coding for Escherichia coli ribonuclease H. J. Biol. Chem. 258, 1276-1281.
8. Kanaya,
S., and Crouch, R. J. (1983) Low levels of RNaseH activity in Escherichia coli FB2 rnh results from a single-base change in
the structural gene of RNase H. J.
Bacteriol. 154, 1021-1026.
9. Kanaya,
S., and Crouch, R. J. (1984) The rnh
gene is essential for growth of Escherichia
coli. Proc. Natl. Acad. Sci. USA,
81, 3447-3451.
10. Emori,
Y., Shiba, T., Kanaya, S., Inouye, S., Yuki, S., and Saigo, K. (1985) The
nucleotide sequence of copia and copla-related RNA in Drosophila virus-like particles. Nature, 316, 773-776.
11. Nagahari,
K., Kanaya, S., Munakata, K., Aoyagi, Y., and Mizushima, S. (1985) Secretion
into the culture medium of a foreign gene product from Escherlchia coll: use of the ompF
gene for secretion of human b-endorphin. EMBO
J. 4, 3589-3592.
12. Sekiguchi,
T., Ortega-Cesana, J., Nosoh, Y., Ohashi, S., Tsuda, K., and Kanaya, S. (1986)
DNA and amino-acid sequences of 3-isopropylmalate dehydrogenase of Bacillus coagulans. Comparison with the
enzymes of Saccharomyces cerevisiae
and Thermus thermophilus. Biochim. Biophys. Acta, 867, 36-44.
13. Kanaya,
S., and Uchida, T. (1986) Comparison of the primary structures of ribonuclease
U2 isoforms. Biochem. J. 240, 163-170.
14. Sato,
S., Nakada, Y., Kanaya, S., and Tanaka, T. (1988) Molecular cloning and
nucleotide sequence of Thermus
thermophllus HB8 trpE and trpG. Biochim. Biophys. Acta, 950,
303-312.
15. Motojima,
K., Kanaya, S., and Goto, S. (1988) Cloning and sequence analysis of cDNA for
rat liver uricase. J. Biol. Chem. 263, 16677-16681.
16. Kanaya,
S., and Yoshida, H. (1988) Photooxidation and modification with 2,3-butanedione
of phosphodiesterase-phosphomonoesterase of Fusarium
moniliforme. Biochem. (Life Sci.
Adv.) 7, 145-148.
17. Xu,
X., Kanaya, S., Koyama, N., Sekiguchi, T., Nosoh, Y., Ohashi, S., and Tsuda, K.
(1989) Tryptic digestion of NADH dehydrogenase from alkalophilic Baclllus. J. Biochem. 105,
626-632.
18. Kanaya,
S., Kohara, A., Miyagawa, M., Matsuzaki, T., Morikawa, K., and Ikehara, M.
(1989) Overproduction and preliminary crystallographic study of ribonuclease H
from Escherlchia coli. J. Biol. Chem. 264, 11546-11549.
19. Kanaya,
S., and Yoshida, H. (1989) PDM phosphatase of Fusarium monlliforme possesses two phosphomonoesterase active sites
with different affinities for p-nitrophenyl
phosphate. Biochem. International, 18, 1121-1127.
20. Kikuchi,
M., Taniyama, Y., Kanaya, S., Takao, T., and Shimonishi, Y. (1990) Occurrence
of S-(1,2-dicarboxyethyl)-cysteine at position 77 in mutant human lysozyme
secreted by Saccharomyces cerevisiae.
Eur. J. Biochem. 187, 315-320.
21. Kanaya,
S., Kohara, A., Miura, Y., Sekiguchi, A., Iwai, S., Inoue, H., Ohtsuka, E., and
Ikehara, M. (1990) Identification of the amino acid residues involved in an
active site of Escherichia coli
ribonuclease H by site-directed mutagenesis. J. Biol. Chem. 265,
4615-4621.
22. Nagayama,
K., Yamazaki, T., Yoshida, M., Kanaya, S., and Nakamura, H. (1990) Combination
of heteronuclear 1H-15N and 1H-13C three dimensional
nuclear magnetic resonance experiments for amide directed sequential assignment
in larger proteins. J. Biochem. 108, 149-152.
23. Katayanagi,
K., Miyagawa, M., Matsushima, M., Ishikawa, M., Kanaya, S.,Ikehara, M.,
Matsuzaki, T., and Morikawa, K. (1990) Three-dimensional structure of
ribonuclease H from E. coli. Nature, 347, 306-309.
24. Kanaya,
S., Kimura, S., Katsuda, C., and Ikehara, M. (1990) Role of cysteine residues
in ribonuclease H from Escherichla coll:
Site-directed mutagenesis and chemical modification. Biochem. J. 271, 59-66.
25. Kanaya,
E., Kanaya, S., and Kikuchi, M. (1990) Mutant human lysozymes with a new
none-native disulfide bridge between residues 64-81: expression, purification
and characterization. Biochem. Biophys.
Res. Commun. 152, 962-967.
26. Kanaya,
S., Katayanagi, K., Morikawa, K., Inoue, H., Ohtsuka, E., and Ikehara, M.
(1991) Effect of the mutagenesis at each of five His residues on enzymatic
activity and stability of ribonuclease H from Escherlchla coli. Eur. J.
Biochem. 198, 437-440.
27. Kanaya,
S., Katsuda, C., Kimura, S., Nakai, T., Kitakuni, E., Nakamura, H., Katayanagi,
K., Morikawa, K., and Ikehara, M. (1991) Stabilization of Escherichia coli ribonuclease H by introduction of an artificial
disulfide bond. J. Biol. Chem. 266, 6038-6044.
28. Kanaya,
S., Katsuda-Nakai, C., and Ikehara, M. (1991) Importance of the positive charge
cluster in Escherlchia coll
ribonuclease HI for the effective binding of the substrate. J. Biol. Chem. 266, 11621-11627.
29. Yamazaki,
T., Yoshida, M., Kanaya, S., Nakamura, H., and Nagayama, K. (1991) Assignments
of backbone 1H,
13C,
and 15N
resonances and secondary structure of ribonuclease H from Escherichia coli using heteronuclear three-dimensional NMR
spectroscopy. Biochemistry, 30, 6036-6047.
30. Nakamura,
H., Oda, Y., Iwai, S., Inoue, H., Ohtsuka, E., Kanaya, S., Kimura, S., Katsuda,
C., Katayanagi, K., Morikawa, K., Miyashiro, H., and Ikehara, M. (1991) How
does ribonuclease H recognize a DNA-RNA hybrid? Proc. Natl. Acad. Sci. USA, 88,
11535-11539.
31. Oda,
Y., Nakamura, H., Kanaya, S., and Ikehara, M. (1991) Binding of metal ions to E. coli RNase H observed by 1H-l5N
heteronuclear two dimensional NMR. J.
Biomol. NMR, 1, 247-255.
32. Oda,
Y., Nakamura, H., Yamazaki, T., Nagayama, K., Yoshida, M., Kanaya, S., and
Ikehara, M. (1992) 1H-NMR studies of deuterated ribonuclease H with
selectively proton-labeled amino acids. J.
Biomol. NMR, 2, 137-147.
33. Katayanagi,
K., Miyagawa, M., Matsushima, M., Ishikawa, M., Kanaya, S., Nakamura, H.,
Ikehara, M., Matsuzaki, T., and Morikawa, K. (1992) Structural details of
ribonuclease H from Escherichia coli as
refined to an atomic resolution. J. Mol.
Biol. 223, 1029-1052.
34. Kanaya,
S., Nakai, C., Konishi, A., Inoue, H., Ohtsuka, E., and Ikehara, M. (1992) A
hybrid ribonuclease H: A novel RNA cleaving enzyme with sequence specific
recognition. J. Biol. Chem. 267, 8492-8498.
35. Kanaya,
S., and Itaya, M. (1992) Expression, purification, and characterization of a
recombinant ribonuclease H from Thermus
thermophilus HB8. J. Biol. Chem. 267, 10184-10192.
36. Kimura,
S., Oda, Y., Nakai, T., Katayanagi, K., Kitakuni, E., Katsuda-Nakai, C.,
Nakamura, H., Ikehara, M., and Kanaya, S. (1992) Effect of the
cavity-modulating mutations on the stability of E. coli ribonuclease HI. Eur.
J. Biochem. 206, 337-343.
37. Kimura,
S., Nakamura, H., Hashimoto, T., Oobatake, M., and Kanaya, S. (1992)
Stabilization of Escherichia coli ribonuclease
HI by strategic replacement of amino acid residues with those from the
thermophilic counterpart. J. Biol. Chem.
267, 21535-21542.
38. Kimura,
S., Kanaya, S., and Nakamura, H. (1992) Thermostabilization of Escheri｣hia coli ribonuclease HI by replacing left-handed
helical Lys95 with Gly or Asn. J. Biol. Chem. 267,
22014-22017.
39. Okumura,
M., Ishikawa, K., Kanaya, S., Itaya, M., and Morikawa, K. (1993)
Crystallization and Preliminary Crystallographic Analysis of Ribonuclease H
from Thermus Thermophilus HB8. Proteins, 15, 108-111.
40. Ishikawa,
K., Kimura, S., Kanaya, S., Morikawa, K., and Nakamura, H. (1993) Structural
study of mutants of Escherichia coli
ribonuclease HI with enhanced thermostability. Protein Eng. 6, 85-91.
41. Ishikawa,
K., Okumura, M., Katayanagi, K., Kimura, S., Kanaya, S., Nakamura, H., and
Morikawa, K. (1993) Crystal structure of ribonuclease H from Thermus thermophilus HB8 refined at 2.8
A resolution. J. Mol. Biol. 230, 529-542.
42. Oda,
Y., Yoshida, M., and Kanaya, S. (1993) Role of histidine 124 in the catalytic
function of ribonuclease HI from Escherichia
coli. J. Biol. Chem. 268, 88-92.
43. Kanaya,
S., Oobatake, M., Nakamura, H., and Ikehara, M. (1993) pH-dependent
thermostabilization of Escherichia coli
ribonuclease HI by histidine to alanine substitutions. J. Biotechnol. 28,
117-136.
44. Ishikawa,
K., Nakamura, H., Morikawa, K., and Kanaya, S. (1993) Stabilization of Escherichia coli ribonuclease HI by
cavity-filling mutations within a hydrophobic core. Biochemistry, 32,
6171-6178.
45. Ishikawa,
K., Nakamura, H., Morikawa, K., Kimura, S., and Kanaya, S. (1993) Cooperative
stabilization of Escherichia coli
ribonuclease HI by insertion of Gly-80b and Gly-77→Ala substitution. Biochemistry,
32, 7136-7142.
46. Katayanagi,
K., Ishikawa, M., Okumura, M., Ariyoshi, M., Kanaya, S., Kawano, Y., Suzuki,
M., Tanaka, I., and Morikawa, K. (1993) Crystal structures of ribonuclease HI
active site mutants from Escherichia coli.
J. Biol. Chem. 268, 22092-22099.
47. Miyamoto,
K., Kanaya, S., Morikawa, K., and Inokuchi, H. (1994) Overproduction,
purification and characterization of ferrochelatase from Escherichia coli. J. Biochem.
115, 545-551.
48. Haruki,
M., Noguchi, E., Nakai, C., Liu, Y-Y., Oobatake, M., Itaya, M., and Kanaya, S.
(1994) Investigating the role of conserved residue Asp134 in Escherichia coli ribonuclease HI by
site-directed random mutagenesis. Eur. J.
Biochem. 220, 623-631.
49. Nakai,
C., Konishi, A., Komatsu, Y., Inoue, H., Ohtsuka, E., and Kanaya, S. (1994)
Sequence-specific cleavage of RNA by a hybrid ribonuclease H. FEBS Lett. 339, 67-72.
50. Oda,
Y., Yamazaki, T., Nagayama, K., Kanaya, S., Kuroda, Y., and Nakamura, H. (1994)
Individual ionization constants of all the carboxyl groups in ribonuclease HI
from Escherichia coli determined by
NMR. Biochemistry, 33, 5275-5284.
51. Uchiyama,
Y., Inoue, H., Ohtsuka, E., Nakai, C., Kanaya, S., Ueno, Y., and Ikehara, M.
(1994) DNA-linked RNase H for site-selective cleavage of RNA. Bioconj. Chem. 5, 327-332.
52. Haruki,
M., Noguchi, E., Akasako, A., Oobatake, M., Itaya, M., and Kanaya, S. (1994) A
novel strategy for stabilization of Escherichia
coli ribonuclease HI involving a screen for an intragenic suppressor of
carboxyl-terminal deletions. J. Biol.
Chem. 269, 26904-26911.
53. Kanaya,
E., Uchiyama, Y., Ohtsuka, E., Ueno, Y., Ikehara, M., and Kanaya, S. (1994)
Kinetic analyses of DNA-linked ribonuclease H with different sizes of DNA. FEBS Lett. 354, 227-231.
54. Ota,
M., Kanaya, S., and Nishikawa, K. (1995) Desk top analysis of the structural stability
of various point mutations introduced into ribonuclease H. J. Mol. Biol. 248,
733-738.
55. Yamasaki,
K., Saito, M., Oobatake, M., and Kanaya, S. (1995) Characterization of internal
motions of Escherichia coli
ribonuclease HI by combination of 15N-NMR relaxation analysis and
molecular dynamics simulation: Examination of dynamic models. Biochemistry, 34, 6587-6601.
56. Akasako,
A., Haruki, M., Oobatake, M., and Kanaya, S. (1995) High resistance of E. coli ribonuclease HI variant with
quintuple thermostabilizing mutations to thermal denaturation, acid
denaturation, and proteolytic degradation. Biochemistry,
34, 8115-8122.
57. Kido,
S., Doi, Y., Kim, F., Morishita, E., Narita, H., Kanaya, S., Ohkubo, T.,
Nishikawa, K., Yao, T., and Ooi, T. (1995) Characterization of vitelline
membrane outer layer protein I, VMO-I: amino acid sequence and structural
stability. J. Biochem. 117, 1183-1191.
58. Kanaya,
E., and Kanaya, S. (1995) Kinetic analysis of E. coli ribonuclease HI using oligomeric DNA/RNA substrates suggests
an alternative mechanism for the interaction between the enzyme and substrate. Eur. J. Biochem. 231, 557-562.
59. Kanaya,
E., and Kanaya, S. (1995) Reconstitution of E.
coli RNase HI from the N-fragment with high helicity and the C-fragment
with a disordered structure. J. Biol.
Chem. 270, 19853-19860.
60. Kanaya,
S., and Uchida, T. (1995) A revised sequence of ribonuclease U2 in a
substrate-binding region. J. Biochem.
118, 681-682.
61. Yamasaki,
T., Kanaya, S., and Oobatake, M. (1995) Heat denaturation and cold denaturation
of Escherichia coli RNase HI
investigated by circular dichroism. Thermochimica
Acta, 267, 379-388.
62. Yamasaki,
K., Ogasahara, K., Yutani, K., Oobatake, M., and Kanaya, S. (1995) Folding
pathway of Escherichia coli
ribonuclease HI: a circular dichroism, fluorescence, and NMR study. Biochemistry, 34, 16552-16562.
63. Yang,
D. W., Yamamoto, K., Kanaya, E., Kanaya, S., and Nagayama, K. (1996)
Characterization of an artificial dimer of ribonuclease H using H1 NMR spectroscopy. J. Biomol. NMR, 7,
29-34.
64. Kashiwagi,
T., Jeanteur, D., Haruki, M., Katayanagi, M., Kanaya, S., and Morikawa, K.
(1996) Proposal of new catalytic roles for two invariant residues in Escherichia coli ribonuclease HI. Protein Eng. 9, 857-867.
65. Fujiwara,
S., Lee, S., Haruki, M., Kanaya, S., Takagi, M., and Imanaka, T. (1996) Unusual
enzyme characteristics of aspartyl-tRNA synthetase from hyperthermophilic
archaeon Pyrococcus sp. KOD1. FEBS Lett. 394, 66-70.
66. Iwai,
S., Wakasa, M., Ohtsuka, E., Kanaya, S., Kidera, A., and Nakamura, H. (1996)
Interaction of the basic protrusion of Escherichia
coli ribonuclease HI with its substrate. J. Mol. Biol. 263,
699-706.
67. Kanaya,
S., Oobatake, M., and Liu, Y-Y. (1996) Thermal stability of E. coli ribonuclease HI and its
active-site mutants in the presence and absence of the Mg2+ ion: proposal of a novel catalytic role
for Glu48.
J. Biol. Chem. 271, 32729-32736.
68. Rashid,
N., Morikawa, M., Nagahisa, K., Kanaya, S., and Imanaka, T. (1997) Characterization
of a RecA/RAD51 homologue from a hyperthermophilic archaeon Pyrococcus sp. KOD1. Nucleic Acids Res. 25, 719-726.
69. Akasako,
A., Haruki, M., Oobatake, M., and Kanaya, S. (1997) Conformational stabilities
of E. coli RNase HI variants with a
series of amino acid substitutions at a cavity within the hydrophobic core. J. Biol. Chem. 272, 18686-18693.
70. Haruki,
M., Noguchi, E., Kanaya, S., and Crouch, R. J. (1997) Kinetic and
stoichiometric analysis with BIAcoreTM for the binding of Escherichia coli ribonuclease HI to
RNA-DNA hybrids. J. Biol. Chem. 272, 22015-22022.
71. Rahman,
R. N. Z. A., Fujiwara, S., Takagi, M., Kanaya, S., and Imanaka, T. (1997)
Effect of heat treatment on proper oligomeric structure formation of
thermostable glutamate dehydrogenase from a hyperthermophilic archaeon. Biophys. Biochem. Res. Comm. 241, 646-652.
72. Yamasaki,
K., Akasako, A., and Kanaya, S. (1998) Structural stability and internal motion
of Escherichia coli ribonuclease HI: 15N
relaxation and hydrogen-deuterium exchange analyses. J. Mol. Biol. 277,
707-722.
73. Kanaya,
S., Koyanagi, T., and Kanaya, E. (1998) An esterase from Escherichia coli with a sequence similarity to hormone-sensitive
lipase. Biochem. J. 332, 75-80.
74. Hirano, N., Haruki, M., Morikawa, M.,
and Kanaya, S. (1998) Stabilization of RNase HI from Thermus thermophilus HB8 by the spontaneous formation of an
intramolecular disulfide bond. Biochemistry,
37, 12640-12648.
75. Haruki, M., Hayashi, K.,
Kochi, T., Muroya, A., Koga, Y., Morikawa, M., Imanaka, T., and Kanaya, S. (1998) Gene
cloning and characterization of recombinant ribonuclease HII from a
hyperthermophilic archaeon. J. Bacteriol.
180, 6207-6214.
76. Koga, Y., Morikawa, M.,
Haruki, M., Nakamura, H., Imanaka, T., and Kanaya, S. (1998) Thermostable
glycerol kinase from a hyperthermophilic archaeon: Gene cloning and
characterization of the recombinant enzyme. Protein
Eng. 11, 1219-1227.
77. Yamasaki,
K., Taniguchi, Y., Takeda, N., Nakano, K., Yamasaki, T., Kanaya, S., and
Oobatake, M. (1998) Pressure-denatured state of Escherichia coli ribonuclease HI as monitored by Fourier transform
infrared and NMR spectroscopies. Biochemistry,
37, 18001-18009.
78. Ohtani, N., Haruki, M.,
Morikawa, M., Crouch, R. J., Itaya, M., and Kanaya, S. (1999) Identification of
the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII
from Bacillus subtilis:
Classification of RNases H into three sub-families. Biochemistry, 38,
605-618.
79. Rashid,
N., Morikawa, M., Kanaya, S., Atomi, H., and Imanaka, T. (1999) A unique DNase
activity shares the active site with ATPase activity of the RecA/Rad51
homologue (Pk-REC) from a
hyperthermophilic archaeon. FEBS Lett.
445, 111-114.
80. Itaya, M., Omori, A., Kanaya,
S., Crouch, R. J., Tanaka, T., and Kondo, K. (1999) Isolation of RNase H genes
that are essential for growth of Bacillus
subtilis 168. J. Bacteriol. 181, 2118-2123.
81. Izumi, M., Fujiwara, S., Takagi, M., Kanaya, S., and Imanaka, T. (1999)
Isolation and characterization of a second subunit of molecular chaperonin from
Pyrococcus kodakaraensis KOD1:
analysis of an ATPase-deficient mutant enzyme. Appl. Environ.
Microbiol. 65, 1801-1805.
82. Haruki, M., Oohashi, Y.,
Mizuguchi, S., Matsuo, Y., Morikawa, M., and Kanaya, S. (1999) Identification
of catalytically essential residues in Escherichia
coli esterase by site-directed mutagenesis. FEBS Lett. 454, 262-266.
83. Okibe,
N., Amada, K., Haruki, M., Imanaka, T., Morikawa, M., and Kanaya, S. (1999)
Gene cloning and characterization of aldehyde dehydrogenase from a petroleum
degrading bacterium strain HD-1. J. Biosci. Bioeng. 88,
7-11.
84. Ohtani, N., Haruki, M.,
Mirikawa, M., and Kanaya, S. (1999) Molecular diversities of RNases H. J. Biosci. Bioeng. 88, 12-19.
85. Huy, N. Q., Jin, S., Amada,
K., Haruki, M., Huu, N. B., Hang, D. T., Ha, D. T. C., Imanaka, T., Morikawa,
M., and Kanaya, S. (1999) Characterization of petroleum degrading bacteria from
oil contaminated sites in Vietnam. J.
Biosci. Bioeng. 88, 100-102.
86. Matsuda, T., Morikawa, M.,
Haruki, M., Higashihata, H., Imanaka, T., and Kanaya, S. (1999) Isolation of
TBP-interacting protein (TIP) from a hyperthermophilic archaeon that inhibits
the binding of TBP to TATA-DNA. FEBS Lett.
457, 38-42.
87. Mizuguchi, S., Amada, K.,
Haruki, M., Imanaka, T., Morikawa, M., and Kanaya, S. (1999) Identification of
the gene encoding esterase, a homolog of hormone-sensitive lipase, from an
oil-degrading bacterium, strain HD-1. J.
Biochem. 126, 731-737.
88. Ohtani,
N., Haruki, M., Muroya, A., Morikawa, M., and Kanaya, S. (2000)
Characterization of ribonuclease HII from Escherichia
coli overproduced in a soluble form. J. Biochem. 127,
895-899.
89. Amada, K., Haruki, M.,
Imanaka, T., Morikawa, M., and Kanaya, S. (2000) Overproduction in E. coli, purification, and
characterization of a family I.3 lipase from Pseudomonas sp. MIS38. Biochim.
Biophys. Acta, 1478, 201-210.
90. Hirano, N., Haruki, M.,
Morikawa, M., and Kanaya, S. (2000) Enhancement of the enzymatic activity of
ribonuclease HI from Thermus thermophilus
HB8 with a suppressor mutation method. Biochemistry,
39, 13285-13294.
91. Haruki, M., Tsunaka, Y.,
Morikawa, M., Iwai, S., and Kanaya, S. (2000) Contribution of phosphate group
of substrate to the catalytic function of Escherichia
coli ribonuclease HI. Biochemistry,
39, 13939-13944.
92. Kwon, H-J, Amada, K, Haruki, M., Morikawa, M., and Kanaya, S. (2000)
Identification of the histidine and
aspartic acid residues essential for enzymatic activity of a family I.3 lipase
by site-directed mutagenesis. FEBS Lett.
483, 139-142.
93. Haruki, M., Nogawa, T.,
Hirano, N., Chon, H, Tsunaka, Y., Morikawa, M., and Kanaya, S. (2000) Efficient cleavage of RNA at high temperatures by a thermostable
DNA-linked ribonuclease H. Protein Eng.
13, 881-886.
94. Kato, T., Haruki, M.,
Imanaka, T., Morikawa, M., and Kanaya, S. (2001) Isolation
and characterization of long-chain-alkane degrading Bacillus thermoleovorans
from deep subterranean petroleum reservoirs. J. Biosci.
Bioeng. 91,
64-70.
95. Kato, T., Miyanaga, A.,
Haruki, M., Imanaka, T., Morikawa, M., and Kanaya, S. (2001) Gene cloning of an alcohol dehydrogenase from thermophilic alkane
degrading Bacillus thermoleovorans
B23. J. Biosci. Bioeng. 91, 100-102.
96. Muroya, A., Tsuchiya, D., Ishikawa, M., Haruki, M., Morikawa, M.,
Kanaya, S., and Morikawa, K. (2001) Catalytic center of an archaeal Type 2
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