大政研究室では、「生物の制御と応用―動物細胞・微生物の産業応用」に関する研究を行っています。

1996年3月

大阪大学工学部応用生物工学科　卒業

1998年3月

大阪大学大学院工学研究科博士前期課程 物質・生命工学専攻　修了

2001年3月

同　上 博士後期課程 物質・生命工学専攻 単位取得退学

2001年5月

学位取得（工学博士）「超好熱始原菌酵素と常温菌酵素の比較研究」

職歴

2011年4月～現在

大阪大学大学院工学研究科　物質生命工学講座　准教授

2007年4月～2011年3月

大阪大学大学院工学研究科　物質生命工学講座　助教

2005年4月～2007年3月

大阪大学大学院工学研究科　物質生命工学講座　助手

2004年7月～2005年3月

大阪大学大学院工学研究科　物質・生命工学専攻　極限生命工学講座　助手

2003年4月～2004年7月

University of Chicago, Department of Biochemistry and Molecular Biology Research associate.

2002年6月～2003年3月

名古屋大学大学院生命農学研究科　博士研究員　　　　　　　　　　　　　　（新エネルギー・産業技術総合開発機構　NEDOフェロー）

2001年4月～2002年5月

名古屋大学大学院生命農学研究科　博士研究員 　　　　　　　　　　　　　　（中部科学技術センター特別研究員）

所属学会

日本生物工学会

　　　

日本農芸化学会

　　　　

日本蛋白質科学会

　　　　　

酵素工学研究会

　

The Protein Society

　　　　　

アジア太平洋プリオン研究会

　　　　

研究業績

【原著論文】（査読付き）
【著書】
【総説、解説】

【原著論文】（査読付き）

95.	Hirata A., Sakudo A., Takano K., Kanaya S. and Koga Y., Effects of Surfactant and a Hyperthermostable Protease on Infectivity of Scrapie-Infected Mouse Brain Homogenate, Journal of Biotechnol and Biomater, 2015, 5(3), 194
94.	Seino S., Watnabe K., Shikakura T., Michika A., Nakagawa T., Koga Y. and Yamamoto T. A., Enzyme Immobilization on Gold/Fe-Oxide Composite Nanoparticles Using a Methionine Tag, Coll and Surf A: Physico and Eng Asp, 2014, in press
93.	You DJ, Jongruja N, Tannous E, Angkawidjaja C, Koga Y, Kanaya S., Structural basis for salt-dependent folding of ribonuclease H1 from halophilic archaeon Halobacterium sp. NRC-1., J Struct Biol. 2014, pii: S1047-8477(14)00141-5.
92.	M, Angkawidjaja C, Koga Y, Kanaya S., Calcium-independent opening of lid1 of a family I.3 lipase by a single Asp to Arg mutation at the calcium-binding site., Protein Eng Des Sel. 2014, 27(5):169-76.
91.	Seino S., Yoshida K., Shikakura T., Watnabe K., Koga Y., Nakagawa T. and Yamamoto T. A., Control the Functionality of Thermoresponsive Enzymes Immobilized onto Magnetic Nanoparticles by the Application of Alternating Magnetic Field., J. Mag. Soc. J., in press
90.	Sulaiman S, You DJ, Kanaya E, Koga Y, Kanaya S. Crystal Structure and Thermodynamic and Kinetic Stability of Metagenome-Derived LC-Cutinase., Biochemistry., 2014, 53(11), 1858-69
89.	Okada K, Angkawidjaja C, Koga Y, Kanaya S., Structural and mechanistic insights into the kynurenine aminotransferase-mediated excretion of kynurenic acid., J Struct Biol. 2014, 185(3):257-66.
88.	Uehara R, Angkawidjaja C, Koga Y, Kanaya S. Formation of the high-affinity calcium binding site in pro-subtilisin E with the insertion sequence IS1 of pro-Tk-subtilisin., Biochemistry. 2013, 52(50):9080-8.
87.	Yuzaki K, Sanda Y, You DJ, Uehara R, Koga Y, Kanaya S., Increase in activation rate of Pro-Tk-subtilisin by a single nonpolar-to-polar amino acid substitution at the hydrophobic core of the propeptide domain., Protein Sci. 2013, 22(12):1711-21.
86.	Permanasari ED, Angkawidjaja C, Koga Y, Kanaya S., Role of N-terminal extension of Bacillus stearothermophilus RNase H2 and C-terminal extension of Thermotoga maritima RNase H2., FEBS J. 2013, 280(20):5065-79.
85.	Koga Y, Tanaka S, Sakudo A, Tobiume M, Aranishi M, Hirata A, Takano K, Ikuta K, Kanaya S., Proteolysis of abnormal prion protein with a thermostable protease from Thermococcus kodakarensis KOD1., Appl Microbiol Biotechnol. 2014, 98(5):2113-20.
84.	Takano K, Aoi A, Koga Y, Kanaya S., Evolvability of thermophilic proteins from archaea and bacteria. Biochemistry. 2013, 52(28):4774-80.
83.	Tadokoro T, Kazama H, Koga Y, Takano K, Kanaya S., Investigating the structural dependence of protein stabilization by amino acid substitution., Biochemistry. 2013, 52(16):2839-47.
82.	Nguyen TN, You DJ, Kanaya E, Koga Y, Kanaya S., Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif., FEBS Lett. 2013, 587(9):1418-23.
81.	Nguyen TN, You DJ, Matsumoto H, Kanaya E, Koga Y, Kanaya S., Crystal structure of metagenome-derived LC11-RNase H1 in complex with RNA/DNA hybrid., J Struct Biol. 2013, 182(2):144-54.
80.	Hirata A, Hori Y, Koga Y, Okada J, Sakudo A, Ikuta K, Kanaya S, Takano K., Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensis in detergents and its ability to degrade the abnormal prion protein., BMC Biotechnol. 2013, 13:19.
79.	Uehara R, Ueda Y, You DJ, Koga Y, Kanaya S., Accelerated maturation of Tk-subtilisin by a Leu→Pro mutation at the C-terminus of the propeptide, which reduces the binding of the propeptide to Tk-subtilisin., FEBS J. 2013;280(4):994-1006
78.	Okada J, Koga Y, Takano K, Kanaya S., Slow unfolding pathway of hyperthermophilic Tk-RNase H2 examined by pulse proteolysis using the stable protease Tk-subtilisin. Biochemistry. 2012, 51(45):9178-91.
77.	Uehara R, Tanaka S, Takano K, Koga Y, Kanaya S., Requirement of insertion sequence IS1 for thermal adaptation of Pro-Tk-subtilisin from hyperthermophilic archaeon., Extremophiles. 2012, 16(6):841-51.
76.	Cheng M, Angkawidjaja C, Koga Y, Kanaya S., Requirement of lid2 for interfacial activation of a family I.3 lipase with unique two lid structures., FEBS J. 2012, 279(19):3727-37.
75.	Okada K, Angkawidjaja C, Koga Y, Takano K, Kanaya S., Characteristic features of kynurenine aminotransferase allosterically regulated by (alpha)-ketoglutarate in cooperation with kynurenine., PLoS One. 2012, 7(7):e40307
74.	Angkawidjaja C, Koga Y, Takano K, Kanaya S.,Structure and stability of a thermostable carboxylesterase from the thermoacidophilic archaeon Sulfolobus tokodaii., FEBS J. 2012 279(17):3071-84.
73.	Jongruja N, You DJ, Angkawidjaja C, Kanaya E, Koga Y, Kanaya S., Structure and characterization of RNase H3 from Aquifex aeolicus., FEBS J. 2012 279(15):2737-53.
72.	Uehara R, Takeuchi Y, Tanaka S, Takano K, Koga Y, Kanaya S., Requirement of Ca(2+) Ions for the Hyperthermostability of Tk-Subtilisin from Thermococcus kodakarensis., Biochemistry. 2012 ,51(26):5369-78.
71.	Nguyen TN, Angkawidjaja C, Kanaya E, Koga Y, Takano K, Kanaya S., Activity, stability, and structure of metagenome-derived LC11-RNase H1, a homolog of Sulfolobus tokodaii RNase H1., Protein Sci. 2012,21(4):553-61.
70.	Budiman C, Tadokoro T, Angkawidjaja C, Koga Y, Kanaya S., Role of polar and nonpolar residues at the active site for PPIase activity of FKBP22 from Shewanella sp. SIB1., FEBS J. 2012, 1742-4658.
69.	Sulaiman S, Yamato S, Kanaya E, Kim JJ, Koga Y, Takano K, Kanaya S., Isolation of a novel cutinase homolog with polyethylene terephthalate-degrading activity from leaf-branch compost by using a metagenomic approach., Appl Environ Microbiol. 2012, 78(5):1556-62.
68.	Kim JJ, Kanaya E, Weon HY, Koga Y, Takano K, Dunfield PF, Kwon SW, KanayaS., Flavobacterium compostarboris sp. nov., isolated from leaf-and-branch compost, and emended descriptions of Flavobacterium hercynium, Flavobacterium resistens and Flavobacterium johnsoniae., Int J Syst Evol Microbiol. 2012, 62(Pt 8):2018-24.
67.	Budiman C, Koga Y, Takano K, Kanaya S., FK506-Binding Protein 22 from a Psychrophilic Bacterium, a Cold Shock-Inducible Peptidyl Prolyl Isomerase with the Ability to Assist in Protein Folding., Int J Mol Sci. 2011, 12, 5261-84.
66.	Budiman C, Angkawidjaja C, Motoike H, Koga Y, Takano K, Kanaya S., Crystal structure of N-domain of FKBP22 from Shewanella sp. SIB1: Dimer dissociation by disruption of Val-Leu knot., Protein Sci., 2011, 20, 755-64.
65.	Miyashita S, Tadokoro T, Angkawidjaja C, You DJ, Koga Y, Takano K, Kanaya S., Identification of the substrate binding site in the N-terminal TBP-like domain of RNase H3., FEBS Lett., 2011, 585, 2313-2317.
64.	Sinsereekul N, Foophow T, Yamanouchi M, Koga Y, Takano K, Kanaya S., An alternative mature form of subtilisin homologue, Tk-SP, from Thermococcus kodakaraensis identified in the presence of Ca2+., FEBS J. 2011, 278, 1901-1911
63.	Tanaka SI, Koga Y, Takano K, Kanaya S., Inhibition of chymotrypsin- and subtilisin-like serine proteases with Tk-serpin from hyperthermophilic archaeon Thermococcus kodakaraensis., Biochim Biophys Acta., 2011,14, 299-307
62.	Takano K, Okamoto T, Okada J, Tanaka S, Angkawidjaja C, Koga Y, Kanaya S., Protein stabilization tag: stabilization by fusion to the C-terminus of hyperthermophile Sulfolobus tokodaii RNase HI., PLoS ONE, 2011, 6, e16226
61.	Iefuji N, Murai R, Maruyama M, Takahashi Y, Sugiyama S, Adachi H, Matsumura H, Murakami S, Inoue T, Mori Y, Koga Y, Takano K, Kanaya S., Laser-induced nucleation in protein crystallization: local increase in protein concentration induced by femtosecond laser irradiation., J. Cryst. Growth, 2011
60.	Kuwahara K, Angkawidjaja C, Koga Y, Takano K, Kanaya S., Importance of an extreme C-terminal motif of a family I.3 lipase for stability., Protein Eng. Des. Sel., 2011, 24, 411-418.
59.	Kim JJ, Jin HM, Lee HJ, Jeon CO, Kanaya E, Koga Y, Takano K, Kanaya S., Flavobacterium banpakuense sp. nov. isolated from leaf-and-branch compost., Int J Syst Evol Microbiol., 2010, 61, 1595-1600.
58.	Sato A, Yokotani A, Tadokoro T, Tanaka S, Angkawidjaja C, Koga Y, Takano K, Kanaya S., Crystal structure of stable protein, CutA1, from a psychrotrophic bacterium Shewanella sp. SIB1., J. Synchrotron Rad., 18, 6-10, 2011
57.	Jongruja N, You DJ, Kanaya E, Koga Y, Takano K, Kanaya S., The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg(2+) -dependent activity., FEBS J., 277, 4474-89, 2010
56.	Okada J, Okamoto T, Mukaiyama A, Tadokoro T, You DJ, Chon H, Koga Y, Takano K, Kanaya S., Evolution and thermodynamics of the slow unfolding of hyperstable monomeric proteins., BMC Evol Biol., 10, 207, 2010
55.	Foophow T, Tanaka S, Angkawidjaja C, Koga Y, Takano K, Kanaya S., Crystal structure of a subtilisin homologue, Tk-SP, from Thermococcus kodakaraensis: requirement of a C-terminal beta-jelly roll domain for hyperstability., J Mol Biol., 400, 865-77, 2010
54.	Angkawidjaja C, Matsumura H, Koga Y, Takano K, Kanaya S., X-ray crystallographic and MD simulation studies on the mechanism of interfacial activation of a family I.3 lipase with two lids., J Mol Biol., 400, 82-95, 2010
53.	Tanaka M, Chon H, Angkawidjaja C, Koga Y, Takano K, Kanaya S., Protein core adaptability: crystal structures of the cavity-filling variants of Escherichia coli RNase HI., Protein Pept Lett., 17, 1163-9, 2010
52.	Kanaya E, Sakabe T, Nguyen NT, Koikeda S, Koga Y, Takano K, Kanaya S., Cloning of the RNase H genes from a metagenomic DNA library: identification of a new type 1 RNase H without a typical active-site motif., J Appl Microbiol., 109, 974-83, 2010
51.	Foophow T, Tanaka S, Koga Y, Takano K, Kanaya S., Subtilisin-like serine protease from hyperthermophilic archaeon Thermococcus kodakaraensis with N- and C-terminal propeptides., Protein Eng Des Sel., 23, 347-55, 2010
50.	Takeuchi Y, Tanaka SI, Matsumura H, Koga Y, Takano K, Kanaya S., Requirement of a Unique Ca(2+)-Binding Loop for Folding of Tk-Subtilisin from a Hyperthermophilic Archaeon. Biochemistry, 48, 10637-43, 2009
49.	Tanaka SI, Matsumura H, Koga Y, Takano K, Kanaya S., Identification of the Interactions Critical for Propeptide-Catalyzed Folding of Tk-Subtilisin., J Mol Biol., 394, 306-19, 2009
48.	Budiman C, Bando K, Angkawidjaja C, Koga Y,Takano K, Kanaya S., Engineering of monomeric FK506-binding protein 22 with peptidyl prolyl cis-trans isomerase. Importance of a V-shaped dimeric structure for binding to protein substrate., FEBS J., 276(15), 4091-101, 2009
47.	Rohman MS, Tadokoro T, Angkawidjaja C, Abe Y, Matsumura H, Koga Y, Takano K, Kanaya S., Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses., FEBS J., 276(2), 603-13, 2009
46.	Takano K, Higashi R, Okada J, Mukaiyama A, Tadokoro T, Koga Y,, Kanaya S., Proline effect on the th ermostability and slow unfolding of a hyperthermophilic protein., J Biochem., 145(1), 79-85, 2009
45.	Kuwahara K, Angkawidjaja C, Matsumura H, Koga Y, Takano K, Kanaya S., Importance of the Ca2+-binding sites in the N-catalytic domain of a family I.3 lipase for activity and stability., Protein Eng Des Sel., 21(12), 737-44, 2008
44.	TanakaS., Takeuchi Y., Matsumura H., Koga Y, Takano K. and Kanaya S., Crystal structure of Tk-subtilisin folded without propeptide: requirement of propeptide for acceleration of folding, FEBS J., 582, 3875-8, 2008
43.	Rohman MS, Koga Y, Takano K, Chon H, Crouch RJ, Kanaya S., Effect of the disease-causing mutations identified in human ribonuclease (RNase) H2 on the activities and stabilities of yeast RNase H2 and archaeal RNase HII., FEBS J., 275(19), 4836-49, 2008
42.	Tadokoro T, Matsushita K, Abe Y, Rohman MS, Koga Y, Takano K, Kanaya S., Remarkable stabilization of a psychrotrophic RNase HI by a combination of thermostabilizing mutations identified by the suppressor mutation method., Biochemistry., 47(31), 8040-7, 2008
41.	Koga Y, Katsumi R, You DJ, Matsumura H, Takano K, Kanaya S., Crystal structure of highly thermostable glycerol kinase from a hyperthermophilic archaeon in a dimeric form., FEBS J., 275(10), 2632-43, 2008
40.	Dong H, Mukaiyama A, Tadokoro T, Koga Y, Takano K, Kanaya S., Hydrophobic effect on the stability and folding of a hyperthermophilic protein., J Mol Biol., 378(1), 264-72, 2008
39.	Pulido MA, Tanaka S, Sringiew C, You DJ, Matsumura H, Koga Y, Takano K, Kanaya S., Requirement of left-handed glycine residue for high stability of the Tk-subtilisin propeptide as revealed by mutational and crystallographic analyses., J Mol Biol., 374(5), 1359-73, 2007
38.	Haruki M, Tanaka M, Motegi T, Tadokoro T, Koga Y, Takano K, Kanaya S., Structural and thermodynamic analyses of Escherichia coli RNase HI variant with quintuple thermostabilizing mutations., FEBS J., 274(22), 5815-25, 2007
37.	Mukaiyama A, Koga Y, Takano K, Kanaya S., Osmolyte effect on the stability and folding of a hyperthermophilic protein., Proteins., 71(1), 110-8, 2007
36.	You DJ, Chon H, Koga Y, Takano K, Kanaya S., Crystal Structure of Type 1 Ribonuclease H from Hyperthermophilic Archaeon Sulfolobus tokodaii: Role of Arginine 118 and C-Terminal Anchoring, Biochemistry., 46(41), 11494-503., 2007
35.	You DJ, Fukuchi S, Nishikawa K, Koga Y, Takano K, Kanaya S., Protein thermostabilization requires a fine-tuned placement of surface charged residues., J Biochem (Tokyo)., 142(4), 507-16, 2007
34.	Tadokoro T, Chon H, Koga Y, Takano K, Kanaya S., Identification of the gene encoding a type 1 RNase H with an N-terminal double-stranded RNA binding domain from a psychrotrophic bacterium., FEBS J., 274(14), 3715-27., 2007
33.	Tadokoro T, You DJ, Abe Y, Chon H, Matsumura H, Koga Y, Takano K, Kanaya S., Structural, thermodynamic, and mutational analyses of a psychrotrophic RNase HI., Biochemistry, 46(25), 7460-8., 2007
32.	Takano K, Katagiri Y, Mukaiyama A, Chon H, Matsumura H, Koga Y, Kanaya S., Conformational contagion in a protein: structural properties of a chameleon sequence., Proteins., 68(3), 617-25., 2007
31.	Pulido MA, Koga Y, Takano K, Kanaya S., Directed evolution of Tk-subtilisin from a hyperthermophilic archaeon: identification of a single amino acid substitution responsible for low-temperature adaptation., Protein Eng Des Sel., 20(3), 143-53., 2007
30.	Katsumi R, Koga Y, You DJ, Matsumura H, Takano K, Kanaya S., Crystallization and preliminary X-ray diffraction study of glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakaraensis., Acta Crystallogr Sect F Struct Biol Cryst Commun., 63(Pt 2), 126-9., 2007
29.	Angkawidjaja C., You DJ., Matsumura H., Kuwahara K., Koga Y., Takano K., Kanaya S., Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation., FEBS Lett., 581(26), 5060-4, 2007
28.	Angkawidjaja C., You DJ., Matsumura H., Koga Y., Takano K., Kanaya S., Extracellular overproduction and preliminary crystallographic analysis of a family I.3 lipase., Acta Cryst F63, 187-9, 2007
27.	Tanaka S, Matsumura H, Koga Y, Takano K, Kanaya S., Four new crystal structures of Tk-subtilisin in unautoprocessed, autoprocessed and mature forms: insight into structural changes during maturation., J Mol Biol., 372(4), 1055-69., 2007
26.	Tanaka S, Saito K, Chon H, Matsumura H, Koga Y, Takano K, Kanaya S. Crystal structure of unautoprocessed precursor of subtilisin from a hyperthermophilic archaeon: evidence for Ca2+-induced folding., J. Biol. Chem., 282(11), 8246-55., 2007
25.	Kashii M, Fujisawa R, Adachi H, Mori Y, Sasaki T, Koga Y, Takano K, Kanaya S, Matsumura H, Inoue T, Murakami S, Sugamoto K, Yoshikawa H., Application of femtosecond laser ablation for detaching grown protein crystals from glass capillary tube., J Biosci Bioeng., 102(4), 372-4., 2006
24.	Mukaiyama A., Haruki M., Ota M., Koga Y., Takano K., Kanaya S., A hyperthermophili protein acquires functio at the cost of stability, Biochemistry, 45, 12673-9, 2006
23.	Tanaka S., Saito K., Chon H., Matsumura H., Koga Y., Takano K., and Kanaya S., Crystallization and preliminary X-ray diffraction study of active-site mutant of pro-Tk-subtilisin from a hyperthermophilic archaeon., Acta. Cryst. F, 62, 902-5, 2006
22.	You D., Chon H., Koga Y., Takano K., Kanaya S., Crystallization and preliminary crystallographic analysis of type1 RNaseH from the hyperthermophilic archaeon sulfolobus tokodaii 7, Acta. Cryst. F, 62, 781-4, 2006
21.	Kakinouchi K., Adachi H., Matsumura H., Inoue T., Murakami S., Mori Y., Koga Y., Takano K., Kanaya S., Effect of ultrasonic irradiation on protein crystallization, J. Cryst. Growth, 292, 437-40, 2006
20.	Angkawidjaja C., Kuwahara K., Omori K., Koga Y., Takano K., Kanaya S., Extracellular secretion of Escherichia coli alkaline phosphatase with a C-terminal tag by typeI secretion system: purification and biochemical characterization Protein Engineering, Design & Screening, 19(7), 337-43 , 2006
19.	Pulido M, Saito K., Tanaka S., Koga Y., Morikawa M., Takano K. and Kanaya S., Ca2-Dependent Maturation of Subtilisin from a Hyperthermophilic Archaeon, Thermococcus kodakaraensis: the Propeptide Is a PotentInhibitor of the Mature Domain but Is Not Required for Its Folding, Applied and Environmental Microbiology, 72(6), 4154–62, 2006
18.	Chon H., Tadokoro T., Ohtani N.,Koga Y., Takano K. and Kanaya S., Identification of RNaseHI from psycrotorophic bacterium, Shewanella sp. SIB1 as a high-activity type RNaseH,, FEBS J., 273, 2264-75, 2006
17.	Cornista J., Koga Y., Takano K. & Kanaya S., Amyloidogenecity and Pitrilysin Sensitivity of a Lysine-free Derivative of Amyloid beta-peptide Cleaved from a Recombinant Fusion Protein, J. Biotechnol., 122, 186-97, 2006
16.	Chon H., Matsumura H., Koga Y., Takano K. & Kanaya S., Crystal Structure and Structure-based Mutational Analyses of RNase HIII from Bacillus stearothermophilus: a New Type 2 RNase H with TBP-like Substrate-binding Domain at the N-terminus, J. Mol. Biol., 356, 165-78, 2006
15.	Takano K., Endo S., Mukaiyama A., Chon H., Matsumura H., Koga Y. & Kanaya S., Structure of Amyloid b Fragments in Aqueous Environments, FEBS J., 273,150-8, 2006
14.	Suzuki Y., Ohnmar Y. W., Koga Y., Takano K. & Kanaya S., Binding analysis of a psychrotrophic FKBP22 to a folding intermediate of protein using surface plasmon resonance,, FEBS Let., 579, 5781-4, 2005
13.	Chon H., Matsumura H., Koga Y., Takano K. & Kanaya S., Crystal Structure of a Human Kynurenine Aminotransferase II Homologue from Pyrococcus horikoshii OT3 at 2.20 angstrome Resolution, Proteins, 61, 685-8, 2005
12.	Angkawidjaja C, Paul A, Koga Y, Takano K, Kanaya S., Importance of a repetitive nine-residue sequence motif for intracellular stability and functional structure of a family I.3 lipase, FEBS Letters, 579, 4707-12, 2005
11.	Kato R, Nakano H, Konishi H, Kato K, Koga Y, Yamane T, Kobayashi T, Honda H., J Novel strategy for protein exploration: high-throughput screening assisted with fuzzy neural network,. Mol. Biol., 351, 683-92 , 2005
10.	Takeuchi K, Kitano H, Adachi H, Mori Y, Sasaki T, Matsumura H, Inoue T, Murakami S, Doi M, Koga Y, Takano K, Kanaya S., Protein crystal growth using laser-processed seed crystals, Jap. J. Appl. Phys., 44(5A), 3177-9, 2005
9.	Chon H, Matsumura H, Shimizu S, Maeda N, Koga Y, Takano K, Kanaya S., Overproduction and preliminary crystallographic study of a human kynurenine aminotransferase II ho,ologue from Pyrococcus horikosii OT3, Acta Cryst., F61, 319-22, 2005
8.	Chon H, Matsumura H, Koga Y, Takano K, Kanaya S., Crystallization and preliminary X-ray diffraction study of thermostable RNaseHII from Bacillus stearothermophilus, Acta Cryst., F61, 293-5, 2005
7.	Koga, Y., Kato, K., Nakano, H. and Yamane, T., Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorialmutation and high-throughput screening using single-molecule PCR and in vitro expression. J. Mol Biol., 331, 585-92, 2003
6.	Koga, Y., Kobayashi, K., Yang, J., Nakano, H., Yamane, T., In vitro construction and screening of Burkhorderia cepacia lipase library by using single-molecule PCR and cell-free protein synthesis., J. Biosci. Bioeng., 94, 84-6, 2002
5.	Yang, J., Koga, Y., Nakano, H. and Yamane, T., Modifying the chain length selectivity of the lipase from Burkholderia cepacia KWI-56 through in vitro combinatorial mutagenesis in the substrate binding site, Protein engineering, 15, 147-52, 2001
4.	Koga, Y., Haruki, M., Morikawa, M., and Kanaya, S., Stabilities of chimeras of hyperthermophilic and mesophilic glycerol kinases constructed by DNA shuffling, J. Biosci. Bioeng., 91, 551-6, 2001
3.	Kannan, Y., Koga, Y., Inoue, Y., Haruki, M., Takagi, M., Imanaka, T., Morikawa, M., and Kanaya, S., Active subtilisin-like protease from a hyperthermophilic archaeon in a form with a putative prosequence, Appl. Environ. Microbiol., 67, 2445-52, 2001
2.	Haruki, M., Hayashi, K., Kouchi, T., Muroya, A., Koga, Y., Morikawa, M., Imanaka, T., Kanaya, S., Cloning of a ribonuclease HII gene from a hyperthermophilic archaeon: Purification and characterization of a recombinant enzyme. , J. Bacteriol., 180, 6207-14, 1998
1.	Koga Y., Morikawa, M., Haruki, M., Nakamura, H., Imanaka, T., Kanaya, S., Thermostable glycerol kinase from a hyperthermophilic archaeon: Cloning of gene, overexpression in E.coli, and characterization of the enzyme. , Protein Eng., 11, 1219-27, 1998
2015年12月更新

【著書】

5.	古賀雄一（コンテンツの分担作製）「実践・化学英語リスニング　生化学編」化学同人 Part 1-Chapter 2, Part3-Chapter6, 2013
4.	荒西睦雄、古賀雄一 (分担執筆)「バイオ消毒剤、洗浄剤(酵素洗浄剤の応用)」、食と健康の高安全化　－殺菌、滅菌、消毒、不活化、有害物質除去技術-、S&T出版　2012, 177-85
3.	古賀雄一、中野秀雄（分担執筆）「酵素の機能改変における結晶構造の役割」、タンパク質結晶の新展開、シーエムシー出版、2008, 251-61
2.	Koga Y., Yamane T., Nakano H.,　（分担執筆） “Creation of novel enantioselective lipases by SIMPLEX”, In vitro transcription and translatio protocols, 2nd edition, Methods in molecular biology, Humana Press, New Jersey, 2007, 165-81
1.	中野秀雄、古賀雄一「無細胞タンパク質合成系によるタンパク質工学」, 化学フロンティアシリーズ⑨コンビナトリアルバイオエンジニアリング, 第20章, 145-149, 2002年
2015年12月更新

【総説、解説】

3.	古賀雄一 「森の中の極限環境微生物－エコ堆肥から見えたこと」、森発見 2008、12, 4-5.
2.	Takano K., Takeda Y., You DJ, Mukaiyama A., Matsumura H., Koga Y. and Kanaya S., Crystal structure of Amyloid beta fragment. New Trends in Alzheimer and Perkinson Related Disorders: ADPD 2007 proceeding
1.	古賀雄一、坂東敬祐、高野和文、金谷茂則「低温菌由来タンパク質の利用」、実用産業情報第39号, 2006, 20-23
2015年12月更新