List of Publications  
 1996---2001
  • Ohtani, N., Haruki, M., Morikawa, M., and Kanaya, S. (2001) Heat labile ribonuclease HI from a psychrotrophic bacterium: gene cloning, characterization, and site-directed mutagenesis. Protein Eng.14, 975-982.

    Amada, K., Kwon, H-J., Haruki, M., Morikawa, M., and Kanaya, S. (2001) Ca2+-induced folding of a family I.3 lipase with repetitive Ca2+ binding motifs at the C-terminus. FEBS Lett. 509, 17-21.

    Kato, T., Haruki, M., Imanaka, T., Morikawa, M., and Kanaya, S. (2001) Isolation and characterization of psychrotrophic bacteria from oil reservoir water and oil sands. Appl. Microbiol. Biotech. 55, 794-800.

    Koga, Y., Haruki, M., Morikawa, M., and Kanaya, S. (2001) Stabilities of chimeras of hyperthermophilic and mesophilic glycerol kinases constructed by DNA shuffling. J. Biosci. Bioeng., 91, 551-556.

    Kannan, Y., Koga, Y., Inoue, Y., Haruki, M., Takagi, M., Imanaka, T., Morikawa, M., and Kanaya, S. (2001) Active subtilisin-like protease from a hyperthermophilic archaeon in a form with a putative prosequence. Appl. Environ. Microbiol., 67, 2445-2552.

    Matsuda, T., Fujikawa, M., Haruki, M., Tang, X.-F., Ezaki, S., Imanaka, T., Morikawa, M., and Kanaya, S. (2001) Interaction of TIP26 from a hyperthermophilic archaeon with TFB/TBP/DNA ternary complex. Extremophiles, 5, 177-182.

    Tsunaka, Y., Haruki, M., Morikawa, M., and Kanaya, S. (2001) Strong nucleic acid binding to the E. coli RNase HI mutant with two arginine residues at the active site. Biochim. Biophys. Acta, 1547, 135-142.

    Muroya, A., Tsuchiya, D., Ishikawa, M., Haruki, M., Morikawa, M., Kanaya, S., and Morikawa, K. (2001) Catalytic center of an archaeal Type 2 Ribonuclease H as revealed by X-ray crystallographic and mutational analyses. Protein Sci., 10, 707-714.

    Kato, T., Miyanaga, A., Haruki, M., Imanaka, T., Morikawa, M., and Kanaya, S. (2001) Gene cloning of an alcohol dehydrogenase from thermophilic alkane degrading Bacillus thermoleovorans B23. J. Biosci. Bioeng. 91, 100-102.

    Kato, T., Haruki, M., Imanaka, T., Morikawa, M., and Kanaya, S. (2001) Isolation and characterization of long-chain-alkane degrading Bacillus thermoleovorans from deep subterranean petroleum reservoirs. J. Biosci. Bioeng. 91, 64-70.

    Takano K, Yamagata Y, Yutani K. (2001) Role of amino acid residues in left-handed helical conformation for the conformational stability of a protein, Proteins, 45, 274-280.

    Takano K, Yutani K. (2001) A new scale for side-chain contribution to protein stability based on the empirical stability analysis of mutant proteins, Protein Eng. 14, 525-528.

    Takano K, Yamagata Y, Yutani K. (2001) Role of non-glycine residues in left-handed helical conformation for the conformational stability of human lysozyme, Proteins, 44, 233-243.

    Funahashi J, Takano K, Yutani K. (2001) Are the parameters of various stabilization factors estimated from mutant human lysozymes compatible with other proteins? Protein Eng. 14, 127-134.

    Takano K, Yamagata Y, Yutani K. (2001) Contribution of polar groups in the interior of a protein to the conformational stability, Biochemistry, 40, 4853-4858.

    Takano K, Funahashi J, Yutani K. (2001) The stability and folding process of amyloidogenic mutant human lysozymes, Eur J Biochem. 268, 155-159.

    Haruki, M., Nogawa, T., Hirano, N., Chon, H, Tsunaka, Y., Morikawa, M., and Kanaya, S. (2000) Efficient cleavage of RNA at high temperatures by a thermostable DNA-linked ribonuclease H. Protein Eng. 13, 881-886.

    Kwon, H-J., Amada, K, Haruki, M., Morikawa, M., and Kanaya, S. (2000) Identification of the histidine and aspartic acid residues essential for enzymatic activity of a family I.3 lipase by site-directed mutagenesis. FEBS Lett. 483, 139-142.

    Haruki, M., Tsunaka, Y., Morikawa, M., Iwai, S., and Kanaya, S. (2000) Catalysis by Escherichia coli ribonuclease HI is facilitated by a phosphate group of the substrate. Biochemistry 39, 13939-13944.

    Hirano, N., Haruki, M., Morikawa, M., & Kanaya, S. (2000) Enhancement of the enzymatic activity of ribonuclease HI from Thermus thermophilus HB8 with a suppressor mutation method. Biochemistry 39, 13285-13294

    Amada, K., Haruki, M., Imanaka, T., Morikawa, M., and Kanaya, S. (2000) Overproduction in E. coli, purification, and characterization of a family I.3 lipase from Pseudomonas sp. MIS38. Biochim. Biophys. Acta 1478, 201-210

    Ohtani, N., Haruki, M., Muroya, A., Morikawa, M., and Kanaya, S. (2000) Characterization of ribonuclease HII from Escherichia coli overproduced in a soluble form. J. Biochem. 127, 895-899

    Funahashi J, Takano K, Yamagata Y, Yutani K. (2000) Role of surface hydrophobic residues in the conformational stability of human lysozyme at three different positions, Biochemistry, 39, 14448-14456.

    Takano K, Tsuchimori K, Yamagata Y, Yutani K. (2000) Contribution of salt bridges near the surface of a protein to the conformational stability, Biochemistry, 39, 12375-12381.

    Takano K, Yamagata Y, Yutani K. (2000) Role of amino acid residues at turns in the conformational stability and folding of human lysozyme, Biochemistry, 39, 8655-8665.

    Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K. (2000) Effect of extra N-terminal residues on the stability and folding of human lysozyme expressed in Pichia pastoris, Protein Eng. 13, 299-307.

    Goda S, Takano K, Yamagata Y, Nagata R, Akutsu H, Maki S, Namba K, Yutani K. (2000) Amyloid protofilament formation of hen egg lysozyme in highly concentrated ethanol solution, Protein Sci. 9, 369-375.

    Morikawa, M., Hirata, Y,. and Imanaka. T,. (2000) A study on the structure-function relationship of lipopeptide biosurfactants. Biochim. Biophys. Acta, 1488, 211-218 (2000).

    Harata, K., Ishii, N., Rashid, N., Morikawa, M., and Imanaka T. (2000). Crystallization and preliminary X-ray study of Pk-REC from a hyperthermophilic archaeon, Pyrococcus kodakaraensis KOD1. Acta Cryst. D56, 648-649.

    Mizuguchi, S., Amada, K., Haruki, M., Imanaka, T., Morikawa, M., & Kanaya, S. (1999) Identification of the gene encoding esterase, a homolog of hormone-sensitive lipase, from an oil-degrading bacterium, strain HD-1. J. Biochem. 126, 731-737

    Matsuda, T., Morikawa, M., Haruki, M., Higashihata, H., Imanaka, T., & Kanaya, S. (1999) Isolation of TBP-interacting protein (TIP) from a hyperthermophilic archaeon that inhibits the binding of TBP to TATA-DNA. FEBS Lett. 457, 38-42

    Huy, N. Q., Jin, S., Amada, K., Haruki, M., Huu, N. B., Hang, D. T., Ha, D. T. C., Imanaka, T., Morikawa, M., and Kanaya, S. (1999) Characterization of petroleum degrading bacteria from oil contaminated sites in Vietnam. J. Biosci. Bioeng. 88, 100-102

    Ohtani, N., Haruki, M., Morikawa, M., & Kanaya, S. (1999) Molecular diversities of RNases H. J. Biosci. Bioeng. 88, 12-19

    Okibe, N., Amada, K., Haruki, M., Imanaka, T., Morikawa, M., and Kanaya, S. (1999) Gene cloning and characterization of aldehyde dehydrogenase from a petroleum degrading bacterium strain HD-1. J. Biosci. Bioeng. 88, 7-11

    Haruki, M., Oohashi, Y., Mizuguchi, S., Matsuo, Y., Morikawa, M., & Kanaya, S. (1999) Identification of catalytically essential residues in Escherichia coli esterase by site-directed mutagenesis. FEBS Lett. 454, 262-266

    Izumi, M., Fujiwara, S., Takagi, M., Kanaya, S., & Imanaka, T. (1999) Isolation and characterization of a second subunit of molecular chaperonin from Pyrococcus kodakaraensis KOD1: analysis of an ATPase-deficient mutant enzyme. Appl. Environ. Microbiol. 65, 1801-1805

    Itaya, M., Omori, A., Kanaya, S., Crouch, R. J., Tanaka, T., & Kondo, K. (1999) Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168. J. Bacteriol. 181, 2118-2123

    Rashid, N., Morikawa, M., Kanaya, S., Atomi, H., & Imanaka, T. (1999) A unique DNase activity shares the active site with ATPase activity of the RecA/Rad51 homologue (Pk-REC) from a hyperthermophilic archaeon. FEBS Lett. 445, 111-114

    Ohtani, N., Haruki, M., Morikawa, M., Crouch, R. J., Itaya, M., & Kanaya, S. (1999) Identification of the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII from Bacillus subtilis: Classification of RNases H into three families. Biochemistry 38, 605-618

    Yamasaki, K., Taniguchi, Y., Takeda, N., Nakano, K., Yamasaki, T., Kanaya, S., & Oobatake, M. (1998) Pressure-denatured state of Escherichia coli ribonuclease HI as monitored by Fourier transform infrared and NMR spectroscopies. Biochemistry 37, 18001-18009.

    Haruki, M., Hayashi, K., Kochi, T., Muroya, A., Koga, Y., Morikawa, M., Imanaka, T., & S. Kanaya. (1998) Gene cloning and characterization of recombinant ribonuclease HII from a hyperthermophilic archaeon. J. Bacteriol. 180, 6207-6214.

    Kaga, Y., Morikawa, M., Haruki, M., Nakamura, H., Imanaka, T., & Kanaya, S. (1998) Thermostable glycerol kinase from a hyperthermophilic archaeon: Gene cloning and characterization of the recombinant enzyme. Protein Engng. in press.

    Hirano, N., Haruki, M., Morikawa, M., & Kanaya, S. (1998) Stabilization of RNase HI from Thermus thermophilus HB8 by the spontaneous formation of an intramolecular disulfide bond. Biochemistry37, 12640-12648.

    Kanaya, S., Koyanagi, T., & Kanaya, E. (1998) An esterase from Escherichia coli with a sequence similarity to hormone-sensitive lipase. Biochem. J. 332, 75-80.

    Yamasaki, K., Akasako-Furukawa, A., & Kanaya, S. (1998) Structural stability and internal motions of Escherichia coli ribonuclease HI: 15N relaxation and hydrogen-deuterium exchange analyses. J. Mol. Biol. 277, 707-722.

    Morikawa, M., Iwasa, T., Yanagida, S., & Imanaka, T. (1998) Production of alkane and alkene from CO2 by a petroleum-degrading bacterium. J. Ferment. Bioeng. 85, 243-245.

    Rahman, R. N. Z. A., Fujiwara, S., Takagi, M., Kanaya, S., & Imanaka, T. (1998) Effect of heat treatment on proper oligomeric structure formation of thermostable glutamate dehydrogenase from a hyperthermophilic archaeon. Biophys. Biochem. Res. Comm. 241, 646-652.

    Kanaya, S. (1998) Enzymatic activity and protein stability of E. coli ribonuclease HI. In Ribonucleases H (Crouch, R. J., & Toulme, J. J., eds), pp. 1-38, INSERM, Paris.

    森川正章、金　沙、金谷茂則、今中忠行（１９９８）“炭酸ガスからの石油生成”日本農芸化学会誌　７２、５３２-５３４．

    金谷茂則（１９９８）“工学研究科物質・生命工学専攻極限生命工学講座”生産技術誌　５０、２８-３３．

    Rashid, N., Morikawa, M., & Imanaka, T. (1997) Gene cloning and characterization of recombinant ribose phosphate pyrophosphokinase from a hyperthermophilic archaeon. J. Ferment. Bioeng. 83, 412-418.

    Haruki, M., Noguchi, E., Kanaya, S., & Crouch, R. J. (1997) Kinetic and stoichiometric analysis with BIAcoreTM for the binding of Escherichia coli ribonuclease HI to RNA-DNA hybrids. J. Biol. Chem. 272, 22015-22022.

    Akasako, A., Haruki, M., Oobatake, M., & Kanaya, S. (1997) Conformational stabilities of E. coli RNase HI variants with a series of amino acid substitutions at a cavity within the hydrophobic core. J. Biol. Chem. 272, 18686-18693.

    Rashid, N., Morikawa, M., Nagahisa, K., Kanaya, S., & Imanaka, T. (1997) Characterization of a RecA/RAD51 homologue from a hyperthermophilic archaeon Pyrococcus sp. KOD1. Nucleic Acids Res., 25 719-726.

    Kanaya, S., Oobatake, M., & Liu, Y-Y. (1996) Thermal stability of E. coli ribonuclease HI and its active-site mutants in the presence and absence of the Mg2+ ion: Proposal of a novel catalytic role for Glu48. J. Biol. Chem. 271, 32729-32736.

    Rashid, N., Morikawa, M., & Imanaka, T. (1996) A RecA/RAD51 homologue from a hyperthermophilic archaeon retains the major RecA domain only. Mol. Gen. Genet. 253, 397-400.

    Morikawa, M., Kanemoto, M., & Imanaka, T. (1996) Biological oxidation of alkane to alkene under anaerobic conditions. J. Ferment. Bioeng. 82, 309-311.

    Iwai, S., Wakasa, M., Ohtsuka, E., Kanaya, S., Kidera, A., & Nakamura, H. (1996) Interaction of the basic protrusion of Escherichia coli ribonuclease HI with its substrate. J. Mol. Biol. 263, 699-706.

    Fujiwara, S., Lee, S., Haruki, M., Kanaya, S., Takagi, M., & Imanaka, T. (1996) Unusual enzyme characteristics of aspartyl-tRNA synthetase from hyperthermophilic archaeon Pyrococcus sp. KOD1. FEBS Lett. 394, 66-70.

    Kashiwagi, T., Jeanteur, D., Haruki, M., Katayanagi, M., Kanaya, S., & Morikawa, K. (1996) Proposal of new catalytic roles for two invariant residues in Escherichia coli ribonuclease HI. Protein Eng., 9, 857-867.

    Healy, J.M., Haruki, M. and Kikuchi, M. (1996) Preferred motif for integlin binding identified using a library of randomized RGD peptides displayed on phage. Protein Peptide Lett., 3, 23-30.

    Yang, D. W., Yamamoto, K., Kanaya, E., Kanaya, S., & Nagayama, K. (1996) Characterization of an artificial dimer of ribonuclease H using H1 NMR spectroscopy. Journal of Biomolecular NMR 7, 29-34.

    金谷茂則（１９９６）“ハイブリッド化による人工RNA制限酵素の創製”生産技術誌　４８、４７ー５０．

    金谷茂則（１９９６）、“狂牛病は人に感染するか？”、日本化学会生体機能関連化学部会ニュースレター　１１、No３、２ー５．

    森川正章､今中忠行（１９９６）、“微生物が人類と地球を救う”、生産技術　４８、５９-６２.