List of Publications  

 

 Before 1995

1.    Kanaya, E., and Kanaya, S. (1995) Reconstitution of E. coli RNase HI from the N-fragment with high helicity and the C-fragment with a disordered structure. J. Biol. Chem. 270, 19853-19860.

2.    Kanaya, E., and Kanaya, S. (1995) Kinetic analysis of E. coli ribonuclease HI using oligomeric DNA/RNA substrates suggests an alternative mechanism for the interaction between the enzyme and substrate. Eur. J. Biochem. 231, 557-562.

3.    Yamasaki, K., Saito, M., Oobatake, M., and Kanaya, S. (1995) Characterization of internal motions of Escherichia coli ribonuclease HI by combination of 15N-NMR relaxation analysis and molecular dynamics simulation: Examination of dynamic models. Biochemistry 34, 6587-6601.

4.    Akasako, A., Haruki, M., Oobatake, M., and Kanaya, S. (1995) High resistance of E. coli ribonuclease HI variant with quintuple thermostabilizing mutations to thermal denaturation, acid denaturation, and proteolytic degradation. Biochemistry 34, 8115-8122.

5.    Kido, S., Doi, Y., Kim, F., Morishita, E., Narita, H., Kanaya, S., Ohkubo, T., Nishikawa, K., Yao, T., and Ooi, T. (1995) Characterization of vitelline membrane outer layer protein I, VMO-I: amino acid sequence and structural stability. J. Biochem. 117, 1183-1191.

6.    Ota, M., Kanaya, S., and Nishikawa, K. (1995) Desk top analysis of the structural stability of various point mutations introduced into ribonuclease H. J. Mol. Biol. 248, 733-738.

7.    Kanaya, S., and Uchida, T. (1995) A revised sequence of ribonuclease U2 in a substrate-binding region. J. Biochem. 118, 681-682.

8.    Yamasaki, T., Kanaya, S., and Oobatake, M. (1995) Heat denaturation and cold denaturation of Escherichia coli RNase HI investigated by circular dichroism. Thermochimica Acta 267, 379-388.

9.    Yamasaki, K., Ogasahara, K., Yutani, K., Oobatake, M., and Kanaya, S. (1995) Folding pathway of Escherichia coli ribonuclease HI: a circular dichroism, fluorescence, and NMR study. Biochemistry 34, 16552-16562.

10.  Miyamoto, K., Kanaya, S., Morikawa, K., and Inokuchi, H. (1994) Overproduction, purification and characterization of ferrochelatase from Escherichia coli. J. Biochem. 115, 545-551.

11.  Haruki, M., Noguchi, E., Nakai, C., Liu, Y-Y., Oobatake, M., Itaya, M., and Kanaya, S. (1994) Investigating the role of conserved residue Asp134 in Escherichia coli ribonuclease HI by site-directed random mutagenesis. Eur. J. Biochem. 220, 623-631.

12.  Nakai, C., Konishi, A., Komatsu, Y., Inoue, H., Ohtsuka, E., and Kanaya, S. (1994) Sequence-specific cleavage of RNA by a hybrid ribonuclease H. FEBS Lett. 339, 67-72.

13.  Oda, Y., Yamazaki, T., Nagayama, K., Kanaya, S., Kuroda, Y., and Nakamura, H. (1994) Individual ionization constants of all the carboxyl groups in ribonuclease HI from Escherichia coli determined by NMR. Biochemistry 33, 5275-5284.

14.  Uchiyama, Y., Inoue, H., Ohtsuka, E., Nakai, C., Kanaya, S., Ueno, Y., and Ikehara, M. (1994) DNA-linked RNase H for site-selective cleavage of RNA. Bioconj. Chem. 5, 327-332.

15.  Haruki, M., Noguchi, E., Akasako, A., Oobatake, M., Itaya, M., and Kanaya, S. (1994) A novel strategy for stabilization of Escherichia coli ribonuclease HI involving a screen for an intragenic suppressor of carboxyl-terminal deletions. J. Biol. Chem. 269, 26904-26911.

16.  Kanaya, E., Uchiyama, Y., Ohtsuka, E., Ueno, Y., Ikehara, M., and Kanaya, S. (1994) Kinetic analyses of DNA-linked ribonuclease H with different sizes of DNA. FEBS Lett. 354, 227-231.

17.  Okumura, M., Ishikawa, K., Kanaya, S., Itaya, M., and Morikawa, K. (1993) Crystallization and Preliminary Crystallographic Analysis of Ribonuclease H from Thermus Thermophilus HB8. Proteins 15, 108-111.

18.  Ishikawa, K., Kimura, S., Kanaya, S., Morikawa, K., and Nakamura, H. (1993) Structural study of mutants of Escherichia coli ribonuclease HI with enhanced thermostability. Protein Eng. 6, 85-91.

19.  Ishikawa, K., Okumura, M., Katayanagi, K., Kimura, S., Kanaya, S., Nakamura, H., and Morikawa, K. (1993) Crystal structure of ribonuclease H from Thermus thermophilus HB8 refined at 2.8 A resolution. J. Mol. Biol. 230, 529-542.

20.  Oda, Y., Yoshida, M., and Kanaya, S. (1993) Role of histidine 124 in the catalytic function of ribonuclease HI from Escherichia coli. J. Biol. Chem. 268, 88-92.

21.  Kanaya, S., Oobatake, M., Nakamura, H., and Ikehara, M. (1993) pH-dependent thermostabilization of Escherichia coli ribonuclease HI by histidine to alanine substitutions. J. Biotechnol. 28, 117-136.

22.  Ishikawa, K., Nakamura, H., Morikawa, K., and Kanaya, S. (1993) Stabilization of Escherichia coli ribonuclease HI by cavity-filling mutations within a hydrophobic core. Biochemistry 32, 6171-6178.

23.  Ishikawa, K., Nakamura, H., Morikawa, K., Kimura, S., and Kanaya, S. (1993) Cooperative stabilization of Escherichia coli ribonuclease HI by insertion of Gly-80b and Gly-77→Ala substitution. Biochemistry 32, 7136-7142.

24.  Katayanagi, K., Ishikawa, M., Okumura, M., Ariyoshi, M., Kanaya, S., Kawano, Y., Suzuki, M., Tanaka, I., and Morikawa, K. (1993) Crystal structures of ribonuclease HI active site mutants from Escherichia coli. J. Biol. Chem. 268, 22092-22099.

25.  Oda, Y., Nakamura, H., Yamazaki, T., Nagayama, K., Yoshida, M., Kanaya, S., and Ikehara, M. (1992) 1H-NMR studies of deuterated ribonuclease H with selectively proton-labeled amino acids. J. Biomol. NMR 2, 137-147.

26.  Katayanagi, K., Miyagawa, M., Matsushima, M., Ishikawa, M., Kanaya, S., Nakamura, H., Ikehara, M., Matsuzaki, T., and Morikawa, K. (1992) Structural details of ribonuclease H from Escherichia coli as refined to an atomic resolution. J. Mol. Biol. 223, 1029-1052.

27.  Kanaya, S., Nakai, C., Konishi, A., Inoue, H., Ohtsuka, E., and Ikehara, M. (1992) A hybrid ribonuclease H: A novel RNA cleaving enzyme with sequence specific recognition. J. Biol. Chem. 267, 8492-8498.

28.  Kanaya, S., and Itaya, M. (1992) Expression, purification, and characterization of a recombinant ribonuclease H from Thermus thermophilus HB8. J. Biol. Chem. 267, 10184-10192.

29.  Kimura, S., Oda, Y., Nakai, T., Katayanagi, K., Kitakuni, E., Katsuda-Nakai, C., Nakamura, H., Ikehara, M., and Kanaya, S. (1992) Effect of the cavity-modulating mutations on the stability of E. coli ribonuclease HI. Eur. J. Biochem. 206, 337-343.

30.  Kimura, S., Nakamura, H., Hashimoto, T., Oobatake, M., and Kanaya, S. (1992) Stabilization of Escherichia coli ribonuclease HI by strategic replacement of amino acid residues with those from the thermophilic counterpart. J. Biol. Chem. 267, 21535-21542.

31.  Kimura, S., Kanaya, S., and Nakamura, H. (1992) Thermostabilization of Escheri｣hia coli ribonuclease HI by replacing left-handed helical Lys95 with Gly or Asn. J. Biol. Chem. 267, 22014-22017.

32.  Kanaya, S., Katayanagi, K., Morikawa, K., Inoue, H., Ohtsuka, E., and Ikehara, M. (1991) Effect of the mutagenesis at each of five His residues on enzymatic activity and stability of ribonuclease H from Escherlchla coli. Eur. J. Biochem. 198, 437-440.

33.  Kanaya, S., Katsuda, C., Kimura, S., Nakai, T., Kitakuni, E., Nakamura, H., Katayanagi, K., Morikawa, K., and Ikehara, M. (1991) Stabilization of Escherichia coli ribonuclease H by introduction of an artificial disulfide bond. J. Biol. Chem. 266, 6038-6044.

34.  Kanaya, S., Katsuda-Nakai, C., and Ikehara, M. (1991) Importance of the positive charge cluster in Escherlchia coll ribonuclease HI for the effective binding of the substrate. J. Biol. Chem. 266, 11621-11627.

35.  Yamazaki, T., Yoshida, M., Kanaya, S., Nakamura, H., and Nagayama, K. (1991) Assignments of backbone 1H, 13C, and 15N resonances and secondary structure of ribonuclease H from Escherichia coli using heteronuclear three-dimensional NMR spectroscopy. Biochemistry 30, 6036-6047.

36.  Nakamura, H., Oda, Y., Iwai, S., Inoue, H., Ohtsuka, E., Kanaya, S., Kimura, S., Katsuda, C., Katayanagi, K., Morikawa, K., Miyashiro, H., and Ikehara, M. (1991) How does ribonuclease H recognize a DNA-RNA hybrid? Proc. Natl. Acad. Sci. USA 88, 11535-11539.

37.  Oda, Y., Nakamura, H., Kanaya, S., and Ikehara, M. (1991) Binding of metal ions to E. coli RNase H observed by 1H-l5N heteronuclear two dimensional NMR. J. Biomol. NMR 1, 247-255.

38.  Kikuchi, M., Taniyama, Y., Kanaya, S., Takao, T., and Shimonishi, Y. (1990) Occurrence of S-(1,2-dicarboxyethyl)-cysteine at position 77 in mutant human lysozyme secreted by Saccharomyces cerevisiae. Eur. J. Biochem. 187, 315-320.

39.  Kanaya, S., Kohara, A., Miura, Y., Sekiguchi, A., Iwai, S., Inoue, H., Ohtsuka, E., and Ikehara, M. (1990) Identification of the amino acid residues involved in an active site of Escherichia coli ribonuclease H by site-directed mutagenesis. J. Biol. Chem. 265, 4615-4621.

40.  Nagayama, K., Yamazaki, T., Yoshida, M., Kanaya, S., and Nakamura, H. (1990) Combination of heteronuclear 1H-15N and 1H-13C three dimensional nuclear magnetic resonance experiments for amide directed sequential assignment in larger proteins. J. Biochem. 108, 149-152.

41.  Katayanagi, K., Miyagawa, M., Matsushima, M., Ishikawa, M., Kanaya, S.,Ikehara, M., Matsuzaki, T., and Morikawa, K. (1990) Three-dimensional structure of ribonuclease H from E. coli. Nature 347, 306-309.

42.  Kanaya, S., Kimura, S., Katsuda, C., and Ikehara, M. (1990) Role of cysteine residues in ribonuclease H from Escherichla coll: Site-directed mutagenesis and chemical modification. Biochem. J. 271, 59-66.

43.  Kanaya, E., Kanaya, S., and Kikuchi, M. (1990) Mutant human lysozymes with a new none-native disulfide bridge between residues 64-81: expression, purification and characterization. Biochem. Biophys. Res. Commun. 152, 962-967.

44.  Xu, X., Kanaya, S., Koyama, N., Sekiguchi, T., Nosoh, Y., Ohashi, S., and Tsuda, K. (1989) Tryptic digestion of NADH dehydrogenase from alkalophilic Baclllus. J. Biochem. 105, 626-632.

45.  Kanaya, S., Kohara, A., Miyagawa, M., Matsuzaki, T., Morikawa, K., and Ikehara, M. (1989) Overproduction and preliminary crystallographic study of ribonuclease H from Escherlchia coli. J. Biol. Chem. 264, 11546-11549.

46.  Kanaya, S., and Yoshida, H. (1989) PDM phosphatase of Fusarium monlliforme possesses two phosphomonoesterase active sites with different affinities for p-nitrophenyl phosphate. Biochem. International 18, 1121-1127.

47.  Sato, S., Nakada, Y., Kanaya, S., and Tanaka, T. (1988) Molecular cloning and nucleotide sequence of Thermus thermophllus HB8 trpE and trpG. Biochim. Biophys. Acta 950, 303-312.

48.  Motojima, K., Kanaya, S., and Goto, S. (1988) Cloning and sequence analysis of cDNA for rat liver uricase. J. Biol. Chem. 263, 16677-16681.

49.  Kanaya, S., and Yoshida, H. (1988) Photooxidation and modification with 2,3-butanedione of phosphodiesterase-phosphomonoesterase of Fusarium moniliforme. Biochem. (Life Sci. Adv.) 7, 145-148.

50.  Sekiguchi, T., Ortega-Cesana, J., Nosoh, Y., Ohashi, S., Tsuda, K., and Kanaya, S. (1986) DNA and amino-acid sequences of 3-isopropylmalate dehydrogenase of Bacillus coagulans. Comparison with the enzymes of Saccharomyces cerevisiae and Thermus thermophilus. Biochim. Biophys. Acta 867, 36-44.

51.  Kanaya, S., and Uchida, T. (1986) Comparison of the primary structures of ribonuclease U2 isoforms. Biochem. J. 240, 163-170.

52.  Emori, Y., Shiba, T., Kanaya, S., Inouye, S., Yuki, S., and Saigo, K. (1985) The nucleotide sequence of copia and copla-related RNA in Drosophila virus-like particles. Nature 316, 773-776.

53.  Nagahari, K., Kanaya, S., Munakata, K., Aoyagi, Y., and Mizushima, S. (1985) Secretion into the culture medium of a foreign gene product from Escherlchia coll: use of the ompF gene for secretion of human b-endorphin. EMBO J. 4, 3589-3592.

54.  Kanaya, S., and Crouch, R. J. (1984) The rnh gene is essential for growth of Escherichia coli. Proc. Natl. Acad. Sci. USA 81, 3447-3451.

55.  Kanaya, S., and Crouch, R. J. (1983) Low levels of RNaseH activity in Escherichia coli FB2 rnh results from a single-base change in the structural gene of RNase H. J. Bacteriol. 154, 1021-1026.

56.  Kanaya, S., and Crouch, R. J. (1983) DNA sequence of the gene coding for Escherichia coli ribonuclease H. J. Biol. Chem. 258, 1276-1281.

57.  Crouch, R. J., Kanaya, S., and Earl, P. L. (1983) A model for the involvement of the small nucleolar RNA (U3) in processing eukaryotic ribosomal RNA. Molec. Biol. Rep. 9, 75-78.

58.  Kanaya, S., and Uchida, T. (1981) An affinity adsorbent, 5'-adenylate-aminohexyl-sepharose. II. Purification and characterization of multi-forms of RNase T2. J. Biochem. 90, 473-481.

59.  Kanaya, S., and Uchida, T. (1981) Purification of ribonuclease T1 by affinity chromatography. J. Biochem. 89, 591-597.

60.  Kanaya, S., and Yoshida, H. (1979) Phosphodiesterase-phosphomono-esterase from Fusarium moniliforme: separation and properties of four isozymes. J. Biochem. 85, 791-797.

61.  Kanaya, S., and Fujimoto, D. (1973) Fragments of polyproline-like structure from a structural protein, cuticlin. J. Mol. Biol. 81, 415-418.

62.  Fujimoto, D., and Kanaya, S. (1973) Cuticlin: A noncollagen structural protein from Ascaris cuticle. Arch. Biochem. Biophys. 157, 1-6.

 

金谷茂則（１９９４）、“リボヌクレアーゼＨ”、蛋白質核酸酵素３９、１１２１ー１１３２．

春木満、金谷茂則（１９９４）、“ランダム変異導入による蛋白質安定化法”、バイオサイエンスとインダストリー５２、３０ー３３.

中村春木、金谷茂則（1993）、”リボヌクレアーゼＨの構造機能相関”、HIV治療薬の開発と蛋白工学（古市泰宏編）、pp.62-76、講談社サイエンテイフィク

金谷茂則（１９９３）、“ＲＮＡの制限酵素が合成されたということですが、従来のＤＮＡ制限酵素とどこが違うのでしょうか。これによってどんなことが可能になるのでしょうか”、化学４８、１０５ー１０７.

金谷茂則（１９９１）、“最新の蛋白質化学について”、有機合成化学４９、７７５ー７７９.

金谷茂則、池原森男（1990）、”蛋白質工学の展望”、タンパク質ハイブリッドIII（稲田祐二、和田博編）、pp. 10-41、共立出版